Spirulina Medium




Spirulina Medium Recipe

Modification of the SAG medium. Alkaline media suitable for LB 2340 Spirulina platensis and LB 2342 Spirulina maxima. In order to prevent the formation of precipitates during autoclaving, two solutions are prepared below as follows:

Directions

For 1 L Total

  • For Liquid Media:
    1. After both solutions 1 and 2 are sterilized, transfer the contents of Solution 2 into the flask containing solution 1, and swirl to mix thoroughly.
    2. Store at refrigerator temperature.
For Liquid:
# Component Amount Stock Solution Concentration Final Concentration
1 Spir solution 1 500 mL/L    
2 Spir solution 2 500 mL/L    
  • For Agar Media:
    1. Prepare three different solutions:
    2. Autoclave all solutions to sterilize.
    3. IMPORTANT: Allow all 3 solutions to cool to ~45-50 °C in a water bath.
    4. Aseptically add 1.0 mL of Vitamin B12 to Solution 2 and mix well.
    5. Combine solution 1 and 2 into solution 3 (agar solution); mix well.
    6. Dispense into sterile tubes or plates.
    7. Allow to cool, then store at refrigerator temperature.
For Agar:
# Component Amount Stock Solution Concentration Final Concentration
1 Spir solution 1 250 mL/L    
2 Spir solution 2 250 mL/L    
3 Spir solution 3 500 mL/L