Spirulina Medium

UTEX sells Spirulina Medium at 1X concentration only. Stock solutions and individual components for this specific medium are currently not available for purchase.

Spirulina Medium

Freshwater Medium | pH>8

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$ 55.00

Spirulina Medium

Modification of the SAG medium. Alkaline media suitable for LB 2340 Spirulina platensis and LB 2342 Spirulina maxima.

Directions For Liquid Media Preparation

In order to prevent the formation of precipitates during autoclaving, two solutions are prepared as follows:

For 1 L Total

After both solutions 1 and 2 are sterilized, transfer the contents of Solution 2 into the flask containing solution 1, and swirl to mix thoroughly.
Store at refrigerator temperature.

#	Component	Amount	Stock Solution Concentration	Final Concentration
1	Spir solution 1	500 mL/L
2	Spir solution 2	500 mL/L

Directions For Agar Media Preparation

In order to prevent the formation of precipitates during autoclaving, three solutions are prepared as follows:

For 1 L Total

After both solutions 1, 2, and 3 are sterilized, allow all solutions to cool to ~45 - 50 °C in a water bath.
Aseptically add 1.0 mL of Vitamin B12 to Solution 2 and mix well.
Combine Solution 1 and 2 into Solution 3 (agar solution); mix well.
Dispense 10 mL into sterile agar tubes.
Allow to cool then store at refrigerator temperature.

#	Component	Amount	Final Concentration
Spir Solution 1
	NaHCO₃ (Fisher S 233)	13.61 g/250 mL dH₂O	162 mM
	Na₂CO₃ (Baker 3604)	4.03 g/250 mL dH₂O	38 mM
	K₂HPO₄ (Sigma P 3786)	0.5 g/250 mL dH₂O	2.9 mM
Spir Solution 2
	NaNO₃ (Fisher BP360-500)	12.5 g/250 mL dH₂O	29.4 mM
	K₂SO₄ (Sigma P 4042)	1 g/250 mL dH₂O	5.74 mM
	NaCl (Fisher S271-500)	1 g/250 mL dH₂O	17.1 mM
	MgSO₄•7H₂O (Sigma 230391)	0.2 g/250 mL dH₂O	0.81 mM
	CaCl₂•2H₂O (Sigma C-3881)	0.04 g/250 mL dH₂O	0.27 mM
	P-IV Metal Solution	6 mL/0.25 L
	Chu Micronutrient Solution	1 mL/0.25 L
	Vitamin B₁₂	1 mL/0.25 L
Spir solution 3
	Agar	15 g/500 mL dH₂O (1.5% w/v)

Spirulina Medium Recipe PDF

Safety Data Sheet (SDS)

FAQs:Algal Culture Media

Important Notes To Consider:

The quality of water, including natural seawater, is important. The term 'dH₂O' generally refers to distilled, deionized, distilled/deionized water, Milli-Q water (Millipore Corp.), etc. Natural seawater and natural freshwater should be obtained from a non-polluted source.
When dissolving chemicals, wait for the first component to dissolve before adding the second. Stirring, and sometimes the addition of heat, is often required to dissolve the chemicals efficiently. Preparation of stock solutions for frequently made algal culture media make preparing media convenient but also necessary to avoid errors from weighing very tiny amounts.
Attention should be given to the pH of the final medium. In most cases, if pH adjustment is required, this occurs before sterilization. Please note that autoclaving removes carbon dioxide from media lacking carbonate stabilizing buffers. This would make the medium very alkaline soon after removal from the autoclave. In these cases, you should wait approximately 24 hours for gaseous equilibrium before inoculating the medium.