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Spirulina Solution 2 Recipe

Directions

For 500 mL Total

  1. To approximately 450 mL of dH2O, add the following components in the order listed while stirring continuously.
  2. Bring total volume to 500 mL with dH2O.
  3. Autoclave medium.
# Component Amount Stock Solution Concentration Final Concentration
1 NaNO3
(Fisher BP360-500)		 2.5 g/500 mL 29.4 mM
2 K2SO4
(Sigma P 4042)		 1 g/500 mL 5.74 mM
3 NaCl
(Fisher S271-500)		 1 g/500 mL 17.1 mM
4 MgSO4•7H2O
(Sigma 230391)		 0.2 g/500 mL 0.81 mM
5 CaCl2•2H2O
(Sigma C-3881)		 0.04 g/500 mL 0.27 mM
6 P-IV Metal Solution 6 mL/0.5 L
7 Chu Micronutrient Solution 1 mL/0.5 L
8 Vitamin B12 1 mL/0.5 L

Note: Final concentration listed is for the stock solution.