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Vitamin B12 Solution Component Recipe

Directions

For 200 mL Total

  1. Prepare 200 mL of HEPES buffer.
  2. Adjust the pH to 7.8.
  3. Add Vitamin B12  wait until fully dissolved.
  4. Sterilize by 0.45 µm Millipore filter. Store in dark at freezer temperature.

** The amount of vitamins added can vary from medium to medium so the final concentration is not listed.

 

# Component Amount Stock Solution Concentration Final Concentration
1 HEPES buffer pH 7.8
(Sigma H-3375)		 2.4 g/200 mL dH2O 50 mM **
2 Vitamin B12
(cyanocobalamin, (Sigma V-6629))		 0.027 g/200 mL dH2O 0.1 mM **

Note: Concentration listed is for the stock solution.