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Chu Micronutrient Solution Recipe

Directions

For 1 L Total

  1. To approximately 900 mL of dH2O add each of the components in the order specified while stirring continuously.
  2. Bring total volume to 1 Liter with dH2O.
  3. Store at refrigerator temperature.
# Component Amount Stock Solution Concentration Final Concentration
1 CuSO4•5H2O
(MCIB 3M11)		 0.02 g/L 0.08 mM
2 ZnSO4•7H2O
(Sigma Z 0251)		 0.044 g/L 0.15 mM
3 CoCl2•6H2O
(Sigma C 3169)		 0.02 g/L 0.084 mM
4 MnCl2•4H2O
(Baker 2540)		 0.012 g/L 0.061 mM
5 Na2MoO4•2H2O
(J.T. Baker 3764)		 0.012 g/L 0.052 mM
6 H3BO3
(Baker 0084)		 0.62 g/L 10 mM
7 Na2EDTA•2H2O
(Sigma ED255)		 0.05 g/L 0.13 mM

Note: Final concentration listed is for the stock solution.