Chu Micronutrient Solution Recipe

Directions

For 1 L Total

  1. To approximately 900 mL of dH2O add each of the components in the order specified while stirring continuously.
  2. Bring total volume to 1 L with dH2O.
  3. Store at refrigerator temperature.
# Component Amount Stock Solution Concentration Final Concentration
1 CuSO45H2O
(MCIB 3M11)
0.02 g/L 0.08 µM
2 ZnSO47H2O
(Sigma Z 0251)
0.044 g/L 0.15 µM
3 CoCl26H2O
(Sigma C-3169)
0.02 g/L 0.084 µM
4 MnCl24H2O
(Baker 2540)
0.012 g/L 0.061 µM
5 Na2MoO42H2O
(J.T. Baker 3764)
0.012 g/L 0.052 µM
6 H3BO3
(Baker 0084)
0.62 g/L 10 µM
7 Na2EDTA‡2H2O
(Sigma ED255)
0.05 g/L 0.13 µM

Note: Final concentration listed is for the stock solution.