P-IV Metal Solution Component Recipe

To approximately 950 mL of dH₂O, add the nutrients in the order listed while stirring continuously.
- Note: The Na₂EDTA should be fully dissolved before adding other components.
Bring total volume to 1 L with dH₂O.
Store at refrigerator temperature.

#	Component	Amount	Final Concentration
1	Na₂EDTA•2H₂O (Sigma ED255)	0.75 g/L	2 mM
2	FeCl₃•6H₂O (Sigma F-1513)	0.097 g/L	0.36 mM
3	MnCl₂•4H₂O (Baker 2540)	0.041 g/L	0.21 mM
4	ZnCl₂ (Sigma Z-0152)	0.005 g/L	0.037 mM
5	CoCl₂•6H₂O (Sigma C-3169)	0.002 g/L	0.0084 mM
6	Na₂MoO₄•2H₂O (J.T. Baker 3764)	0.004 g/L	0.017 mM