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Spirulina Solution 1 Recipe

Directions

For 500 mL Total

  1. To approximately 450 mL of dH2O, add the following components in the order listed while stirring continuously.
  2. Bring total volume to 500 mL with dH2O.
  3. Autoclave medium.
# Component Amount Stock Solution Concentration Final Concentration
1 NaHCO3
(Fisher S 233)		 13.61 g/500 mL dH2O 162 mM
2 Na2CO3
(Baker 3604)		 4.03 g/500 mL dH2O 38 mM
3 K2HPO4
(Sigma P 3786)		 0.5 g/500 mL dH2O 2.9 mM

Note: Final concentration listed is for the stock solution.