Spirulina Solution 1 Recipe

Directions

For 500 mL Total

To approximately 450 mL of dH₂O, add the following components in the order listed while stirring continuously.
Bring total volume to 500 mL with dH₂O.
Autoclave medium.

#
1
Component	NaHCO₃ (Fisher S 233)
Amount	13.61 g/500 mL dH₂O
Final Concentration	162 mM
2
Component	Na₂CO₃ (Baker 3604)
Amount	4.03 g/500 mL dH₂O
Final Concentration	38 mM
3
Component	K₂HPO₄ (Sigma P 3786)
Amount	0.5 g/500 mL dH₂O
Final Concentration	2.9 mM

#
1
Component	NaHCO₃ (Fisher S 233)
Amount	13.61 g/500 mL dH₂O
Final Concentration	162 mM
2
Component	Na₂CO₃ (Baker 3604)
Amount	4.03 g/500 mL dH₂O
Final Concentration	38 mM
3
Component	K₂HPO₄ (Sigma P 3786)
Amount	0.5 g/500 mL dH₂O
Final Concentration	2.9 mM

#	Component	Amount	Stock Solution Concentration	Final Concentration
1	NaHCO₃ (Fisher S 233)	13.61 g/500 mL dH₂O		162 mM
2	Na₂CO₃ (Baker 3604)	4.03 g/500 mL dH₂O		38 mM
3	K₂HPO₄ (Sigma P 3786)	0.5 g/500 mL dH₂O		2.9 mM

Note: Final concentration listed is for the stock solution.