10L Media Kit: MES-Volvox Medium
$ 170.00 $ 550.00
10L Media Kit: MES-Volvox Medium
General purpose medium for freshwater strains, especially those requiring ammonium. Suitable for xenic and axenic cultures. Modification of Volvox Medium. MES buffer is used in place of glycylglycine, and ammonium chloride is added as a second nitrogen source.
Media kit includes all the necessary chemical components to prepare 10 Liters of MES-Volvox Medium. All UTEX Media Kit solutions are shipped sterile and should be handled aseptically to avoid contamination.
What is a media kit? How is it different than stock solutions?
UTEX Media Kits come with pre-measured, ready-to-use components. Unlike stock solutions, there is no need to prepare and/or dilute concentrated reagents for each individual chemical at precise ratios. Simply follow the included instructions to create the large volume of media—precision without the hassle of extra preparation.
Do the media kits include stock solutions?
No. UTEX Media Kits do not include concentrated stock solutions for each individual chemical component of a medium. Compatible chemicals have been pre-measured and combined to not only make preparation easier, but also reduce packaging and shipping costs.
What if I want individual stock solutions?
Each culture medium page includes a recipe that, when applicable, provides instructions for preparing stock solutions and how much to add. Because some components aren’t suitable for stock solutions, related instructions are provided only when applicable.
Looking for MES-Volvox Medium in 1-Liter and test tube volumes?
Medium At A Glance |
|
| Water Type | Freshwater |
| pH of Medium | 6<pH<8 |
| Contains | Micronutrients, NH4, NO3, Biotin, Vitamin B12 |
Directions
For 10L Total Volume
- Obtain and wear the appropriate PPE.
- To approximately 9.7 Liters (9,700 mL) of dH2O, add the first three solutions (Components #1-3) in order while stirring.
- Bring total volume to 10 Liters (10,000 mL) with dH2O.
- Cover and autoclave or filter-sterilize if non-sterile water was used.
- When cooled, aseptically add Component #4.
- Store at refrigerator temperatures.
Note: When combined this gives a pH of 6.5 before autoclaving. Both the standard medium and medium made from the 10L Media Kit show a pH of 6.5 - 6.6 after autoclaving.
| Chemical | Final Concentration |
|---|---|
| Ca(NO3)2•4H2O | 0.5 mM |
| MgSO4•7H2O | 0.16 mM |
| Na2glycerophosphate•5H2O | 0.16 mM |
| KCl | 0.67 mM |
| NH4Cl | 0.5 mM |
| MES | 10 mM |
| P-IV Metal Solution | |
| Vitamin B12 | |
| Biotin Vitamin Solution |
Important Notes To Consider:
- The quality of water, including natural seawater, is important. The term 'dH2O' generally refers to distilled, deionized, distilled/deionized water, Milli-Q water (Millipore Corp.), etc. Natural seawater and natural freshwater should be obtained from a non-polluted source.
- When dissolving chemicals, wait for the first component to dissolve before adding the second. Stirring, and sometimes the addition of heat, is often required to dissolve the chemicals efficiently. Preparation of stock solutions for frequently made algal culture media make preparing media convenient but also necessary to avoid errors from weighing very tiny amounts.
- Attention should be given to the pH of the final medium. In most cases, if pH adjustment is required, this occurs before sterilization. Please note that autoclaving removes carbon dioxide from media lacking carbonate stabilizing buffers. This would make the medium very alkaline soon after removal from the autoclave. In these cases, you should wait approximately 24 hours for gaseous equilibrium before inoculating the medium.
