UTEX sells Volvox Medium at 1X concentration only. Stock solutions and individual components for this specific medium are currently not available for purchase.


Volvox Medium



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Volvox Medium

Modification of the original recipe. Suitable for most axenic strains of Volvox and some of Eudorina and Pandorina. Not suitable for xenic cultures. For additional modifications of Volvox Medium, see HEPES-volvox, MES-volvox, P49, and Volvox-Dextrose.

Directions

For 1 L Total
  1. To approximately 950 mL of dH2O, add the following components in the order listed (except vitamins) while stirring continuously.
  2. Adjust the pH to 8.0
  3. Bring total volume to 1 L with dH2O.
    • For 1.5% agar medium add 15 grams of agar into the flask; do not mix.
  4. Cover and autoclave medium.
  5. Allow to cool and add vitamins.
    • For agar medium add vitamins, mix, and dispense before agar solidifies.

# Component Amount Stock Solution Concentration Final Concentration
1 Ca(NO3)24H2O
(Sigma C 5676)
1 mL/L 11.8 g/100 mL dH2O 0.5 mM
2 MgSO47H2O
(Sigma 230391)
1 mL/L 4 g/100 mL dH2O 0.16 mM
3 Na2glycerophosphate5H2O
(Sigma G 6501 )
1 mL/L 5 g/100 mL dH2O 0.16 mM
4 KCl
(Fisher P 217)
1 mL/L 5 g/100 mL dH2O 0.67 mM
5 Glycylglycine
(Sigma G 1002)
10 mL/L 5 g/100 mL dH2O 3.8 mM
6 P-IV Metal Solution 6 mL/L
7 Vitamin B12 1 mL/L
8 Biotin Vitamin Solution 1 mL/L

Important Notes To Consider:

  • The quality of water, including natural seawater, is important. The term 'dH2O' generally refers to distilled, deionized, distilled/deionized water, Milli-Q water (Millipore Corp.), etc. Natural seawater and natural freshwater should be obtained from a non-polluted source.
  • When dissolving chemicals, wait for the first component to dissolve before adding the second. Stirring, and sometimes the addition of heat, is often required to dissolve the chemicals efficiently. Preparation of stock solutions for frequently made algal culture media make preparing media convenient but also necessary to avoid errors from weighing very tiny amounts.
  • Attention should be given to the pH of the final medium. In most cases, if pH adjustment is required, this occurs before sterilization. Please note that autoclaving removes carbon dioxide from media lacking carbonate stabilizing buffers. This would make the medium very alkaline soon after removal from the autoclave. In these cases, you should wait approximately 24 hours for gaseous equilibrium before inoculating the medium.