Modification of the original recipe. Suitable for most axenic strains of Volvox and some of Eudorina and Pandorina. Not suitable for xenic cultures. For additional modifications of Volvox Medium, see HEPES-volvox, MES-volvox, P49, and Volvox-Dextrose.
# | |
---|---|
1 | |
Component | Ca(NO3)2•4H2O (Sigma C 5676) |
Amount | 1 mL/L |
Stock Solution Concentration | 11.8 g/100 mL dH2O |
Final Concentration | 0.5 mM |
2 | |
Component | MgSO4•7H2O (Sigma 230391) |
Amount | 1 mL/L |
Stock Solution Concentration | 4 g/100 mL dH2O |
Final Concentration | 0.16 mM |
3 | |
Component | Na2glycerophosphate•5H2O (Sigma G 6501 ) |
Amount | 1 mL/L |
Stock Solution Concentration | 5 g/100 mL dH2O |
Final Concentration | 0.16 mM |
4 | |
Component | KCl (Fisher P 217) |
Amount | 1 mL/L |
Stock Solution Concentration | 5 g/100 mL dH2O |
Final Concentration | 0.67 mM |
5 | |
Component | Glycylglycine (Sigma G 1002) |
Amount | 10 mL/L |
Stock Solution Concentration | 5 g/100 mL dH2O |
Final Concentration | 3.8 mM |
6 | |
Component | P-IV Metal Solution |
Amount | 6 mL/L |
7 | |
Component | Vitamin B12 |
Amount | 1 mL/L |
8 | |
Component | Biotin Vitamin Solution |
Amount | 1 mL/L |
# | Component | Amount | Stock Solution Concentration | Final Concentration |
---|---|---|---|---|
1 | Ca(NO3)2•4H2O (Sigma C 5676) |
1 mL/L | 11.8 g/100 mL dH2O | 0.5 mM |
2 | MgSO4•7H2O (Sigma 230391) |
1 mL/L | 4 g/100 mL dH2O | 0.16 mM |
3 | Na2glycerophosphate•5H2O (Sigma G 6501 ) |
1 mL/L | 5 g/100 mL dH2O | 0.16 mM |
4 | KCl (Fisher P 217) |
1 mL/L | 5 g/100 mL dH2O | 0.67 mM |
5 | Glycylglycine (Sigma G 1002) |
10 mL/L | 5 g/100 mL dH2O | 3.8 mM |
6 | P-IV Metal Solution | 6 mL/L | ||
7 | Vitamin B12 | 1 mL/L | ||
8 | Biotin Vitamin Solution | 1 mL/L |
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