Volvox Medium



Volvox Medium Recipe

Modification of the original recipe. Suitable for most axenic strains of Volvox and some of Eudorina and Pandorina. Not suitable for xenic cultures. For additional modifications of Volvox Medium, see HEPES, MES-Volvox, P49, and Volvox-Dextrose.

 

Directions

For 1 L Total

  1. To approximately 950 mL of dH2O, add the following components in the order listed (except vitamins) while stirring continuously.
  2. Adjust the pH to 8.0
  3. Bring total volume to 1 L with dH2O.
    • *For 1.5% agar medium add 15 grams of agar into the flask; do not mix.
  4. Cover and autoclave medium.
  5. Allow to cool and add vitamins.
    • *For agar medium add vitamins, mix, and dispense before agar solidifies.

# Component Amount Stock Solution Concentration Final Concentration
1 Ca(NO3)2·4H2O (Sigma C 5676) 1 mL/L 11.8 g/100 mL dH2O 0.5 mM
2 MgSO4·7H2O (Sigma 230391) 1 mL/L 4 g/100 mL dH2O 0.16 mM
3 Na2glycerophosphate.5H2O (Sigma G 6501 ) 1 mL/L 5 g/100 mL dH2O 0.16 mM
4 KCl (Fisher P 217) 1 mL/L 5 g/100 mL dH2O 0.67 mM
5 Glycylglycine (Sigma G 1002) 10 mL/L 5 g/100 mL dH2O 3.8 mM
6 P-IV Metal Solution 6 mL/L    
7 Vitamin B12 1 mL/L    
8 Biotin Vitamin Solution 1 mL/L