MES-Volvox Medium



MES-Volvox Medium Recipe

General purpose medium for freshwater strains, especially those requiring ammonium. Suitable for xenic and axenic cultures. Modified Volvox Medium.

 

Directions

For 1 L Total

  1. To approximately 950 mL of dH2O, add each of the components in the order specified (except vitamins) while stirring continuously.
  2. Adjust the pH to 6.7
  3. Bring the total volume to 1 L with dH2O.
    • *For 1.5% agar medium add 15 grams of agar into the flask; do not mix.
  4. Cover and autoclave medium.
  5. When cooled, add vitamins.
    • *For agar medium add vitamins, mix, and dispense before agar solidifies.
  6. Store at refrigerator temperature.

# Component Amount Stock Solution Concentration Final Concentration
1 Ca(NO3)2·4H2O (Sigma C 5676) 1 mL/L 11.8 g/100 mL dH2O 0.5 mM
2 MgSO4·7H2O (Sigma 230391) 1 mL/L 4 g/100 mL dH2O 0.16 mM
3 Na2glycerophosphate.5H2O (Sigma G 6501 ) 1 mL/L 5 g/100 mL dH2O 0.16 mM
4 KCl (Fisher P 217) 1 mL/L 5 g/100 mL dH2O 0.67 mM
5 MES (Sigma M-8250) 1.95 g/L   10 mM
6 P-IV Metal Solution 6 mL/L    
7 NH4Cl (Fisher A 649-500) 1 mL/L 2.67 g/100 mL dH2O 0.5 mM
8 Vitamin B12 1 mL/L    
9 Biotin Vitamin Solution 1 mL/L