MES-Volvox Medium



MES-Volvox Medium Recipe

Directions 
General purpose medium for freshwater strains, especially those requiring ammonium. Suitable for xenic and axenic cultures. Modified volvox medium.

For 1 L Total

1. To approximately 950 mL of dH2O, add each of the components in the order specified (except vitamins) while stirring continuously.
2. Adjust the pH to 6.7.
3. Bring the total volume to 1 L with dH2O.
*For 1.5% agar medium add 15 g of agar into the flask; do not mix.
4. Cover and autoclave medium.
5. When cooled add vitamins.
*For agar medium add vitamins, mix, and dispense before agar solidifies.
6. Store at refrigerator temperature.

# Component Amount Stock Solution
Concentration
Final Concentration
1 Ca(NO3)2·4H2O (Sigma C 5676) 1 mL/L 11.8 g/100 mL dH20 0.5 mM
2 MgSO4·7H2O (Sigma 230391) 1 mL/L 4 g/100 mL dH20 0.16 mM
3 Na2glycerophosphate.5H2O (Sigma G 6501 ) 1 mL/L 5 g/100 mL dH20 0.16 mM
4 KCl (Fisher P 217) 1 mL/L 5 g/100 mL dH20 0.67 mM
5 MES (Sigma M-8250) 1.95 g/L   10 mM
6 P-IV Metal Solution 6 mL/L    
7 NH4Cl (Fisher A 649-500) 1 mL/L 2.67 g/100 mL dH20 0.5 mM
8 Vitamin B12 1 mL/L    
9 Biotin Vitamin Solution 1 mL/L