10L Media Kit: Bristol Medium
$ 155.00 $ 175.00
10L Media Kit: Bristol Medium
H.C. Bold's modification of Bristol's recipe (Bold 1949). General purpose freshwater medium that lacks vitamins and is the essential base component for other media such as Bold 3N and Modified Bold 3N Media (see table below).
Media kit includes all the necessary chemical components to prepare 10 Liters of Bristol Medium. All UTEX Media Kit solutions are shipped sterile and should be handled aseptically to avoid contamination.
What is a media kit? How is it different than stock solutions?
UTEX Media Kits come with pre-measured, ready-to-use components. Unlike stock solutions, there is no need to prepare and/or dilute concentrated reagents for each individual chemical at precise ratios. Simply follow the included instructions to create the large volume of media—precision without the hassle of extra preparation.
Do the media kits include stock solutions?
No. UTEX Media Kits do not include concentrated stock solutions for each individual chemical component of a medium. Compatible chemicals have been pre-measured and combined to not only make preparation easier, but also reduce packaging and shipping costs.
What if I want individual stock solutions?
Each culture medium page includes a recipe that, when applicable, provides instructions for preparing stock solutions and how much to add. Because some components aren’t suitable for stock solutions, related instructions are provided only when applicable.
Looking for Bristol Medium in 1-Liter and test tube volumes?
Media That Use Bristol As Base Component
| Bristol Medium | Bold 1NV Medium | Bold 3N Medium | Modified Bold 3N Medium | Bristol-NaCl Medium |
| Bold Basal Medium | Botryococcus Medium | Proteose Medium | Soilwater Extract Medium | Trebouxia Medium |
Available Bristol Media Kit Upgrades
For similar chemical components but with 3X the nitrogen and the addition of vitamins, view the available Bold 3N and Modified Bold 3N upgrades for this kit. You can purchase the Bristol Media Kit with an upgrade at a discount using the 'Options' dropdown at the top of the page. Media Upgrade Kits are also available to purchase separately on their respective pages.
Medium At A Glance
-
Water Type Freshwater
pH of Medium 6<ph<8
Contains NO3
Directions
For 10L Bristol Medium Total Volume
- Obtain and wear the appropriate PPE.
- To approximately 8.4 Liters (8,400 mL) of dH2O, carefully add the entire bottle of Bristol solution while stirring.
- Bring total volume to 10 Liters (10,000 mL) with dH2O.
- Cover and autoclave or filter-sterilize if non-sterile water was used.
- Store at refrigerator temperatures.
| Chemical |
Final Concentration |
|---|---|
| NaNO3 | 2.94 mM |
| CaCl2•2H2O | 0.17 mM |
| MgSO4•7H2O | 0.3 mM |
| K2HPO4 | 0.43 mM |
| KH2PO4 | 1.29 mM |
| NaCl | 0.43 mM |
Important Notes To Consider:
- The quality of water, including natural seawater, is important. The term 'dH2O' generally refers to distilled, deionized, distilled/deionized water, Milli-Q water (Millipore Corp.), etc. Natural seawater and natural freshwater should be obtained from a non-polluted source.
- When dissolving chemicals, wait for the first component to dissolve before adding the second. Stirring, and sometimes the addition of heat, is often required to dissolve the chemicals efficiently. Preparation of stock solutions for frequently made algal culture media make preparing media convenient but also necessary to avoid errors from weighing very tiny amounts.
- Attention should be given to the pH of the final medium. In most cases, if pH adjustment is required, this occurs before sterilization. Please note that autoclaving removes carbon dioxide from media lacking carbonate stabilizing buffers. This would make the medium very alkaline soon after removal from the autoclave. In these cases, you should wait approximately 24 hours for gaseous equilibrium before inoculating the medium.
