Trebouxia Medium

Trebouxia Medium Recipe

Modification of Ahmadjian's medium for the phycobionts of lichens. Suitable for most axenic cultures of Trebouxia, Pseudotrebouxia, Trentepohlia and Cephaleuros.


For 1 L Total

  1. Prepare 1 L of Bristol Medium. Transfer approximately 825 mL to a 2-L Erlenmeyer flask. Save the remaining Solution.
  2. To approximately 825 mL of Bristol Medium, add each of the components in the order specified while stirring continuously.
  3. Bring total volume to 1 L with the Bristol Medium saved from step 1.
    • *For 1.5% (w/v) agar medium: add 15 g of agar into the flask; do not mix.
  4. Cover and autoclave medium. 
  5. Store at refrigerator temperature.

# Component Amount Stock Solution Concentration Final Concentration
1 Bristol Medium 825 mL/L
2 Soilwater: GR+ Medium 140 mL/L of supernatant
3 Proteose Peptone (BD 211684) 10 g/L
4 Glucose (BACTO-Dextrose of Sigma D 9634) 20 g/L 0.11 M