Trebouxia Medium
Modification of Ahmadjian's medium for the phycobionts of lichens. Suitable for most axenic cultures of Trebouxia, Pseudotrebouxia, Trentepohlia and Cephaleuros.
Directions
For 1 L Total
- Prepare 1 L of Bristol Medium. Transfer approximately 825 mL to a 2-L Erlenmeyer flask. Save the remaining Solution.
- To approximately 825 mL of Bristol Medium, add each of the components in the order specified while stirring continuously.
- Bring total volume to 1 L with the Bristol Medium saved from step 1.
- For 1.5% (w/v) agar medium: add 15 g of agar into the flask; do not mix.
- Cover and autoclave medium.
- Store at refrigerator temperature.
| # |
|---|
| 1 |
|---|
| Component | Bristol Medium |
| Amount | 825 mL/L |
| 2 |
|---|
| Component | Soilwater: GR+ Medium |
| Amount | 140 mL/L |
| 3 |
|---|
| Component | Proteose Peptone
(BD 211684) |
| Amount | 10 g/L |
| 4 |
|---|
| Component | Glucose
(BACTO-dextrose or Sigma D 9634) |
| Amount | 20 g/L |
| Final Concentration | 0.11 M |
| # |
Component |
Amount |
Stock Solution Concentration |
Final Concentration |
| 1 |
Bristol Medium |
825 mL/L |
|
|
| 2 |
Soilwater: GR+ Medium |
140 mL/L |
|
|
| 3 |
Proteose Peptone
(BD 211684) |
10 g/L |
|
|
| 4 |
Glucose
(BACTO-dextrose or Sigma D 9634) |
20 g/L |
|
0.11 M |
