Bristol Medium

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Bristol Medium Recipe

H.C. Bold's modification of Bristol's recipe (Bold 1949). General purpose freshwater medium and as bristol's solution, an essential component of other media-- see Bold 1NV Medium, Bold 3N Medium, Bristol-NaCl Medium, LDM Medium, Proteose Medium, Soil Extract Medium, and Trebouxia Medium.

Directions

For 1 L Total

  1. To approximately 900 mL of dH2O, add each of the components in the order specified while stirring continuously.
  2. Bring total volume to 1 L with dH2O.
    • *For 1.5% (w/v) agar medium: add 15 g of agar into the flask; do not mix.
  3. Cover and autoclave medium. 
  4. Store at refrigerator temperature.

# Component Amount Stock Solution Concentration Final Concentration
1 NaNO3 (Fisher BP360-500) 10 mL/L 10 g/400mL dH2O 2.94 mM
2 CaCl2·2H2O (Sigma C-3881) 10 mL/L 1 g/400mL dH2O 0.17 mM
3 MgSO4·7H2O (Sigma 230391) 10 mL/L 3 g/400mL dH2O 0.3 mM
4 K2HPO4 (Sigma P 3786) 10 mL/L 3 g/400mL dH2O 0.43 mM
5 KH2PO4 (Sigma P 0662) 10 mL/L 7 g/400mL dH2O 1.29 mM
6 NaCl (Fisher S271-500) 10 mL/L 1 g/400mL dH2O 0.43 mM