Bold 1NV Medium

ATTENTION:

Due to the upcoming 2018 Winter Holiday closures, orders placed after the stated deadlines will not ship until 2019 (review expected shipping estimates).




Bold 1NV Medium Recipe

Modification of Bold's recipe. General purpose freshwater medium used for xenic cultures.

Directions

For 1 L Total

  1. To approximately 900 mL of dH2O, add each of the components in the order specified (except vitamins) while stirring continuously.
  2. Bring the total volume to 1 L with dH2O.
    • *For 1.5% (w/v) agar medium, add 15 g of agar into the flaskl; do not mix.
  3. Cover and autoclave medium.
  4. When cooled add vitamins.
    • *For agar medium add vitamins, mix, and dispense before agar solidifies.
  5. Store at refrigerator temperature.

# Component Amount Stock Solution Concentration Final Concentration
1 NaNO3 (Fisher BP360-500) 10 mL/L 10 g/400mL dH2O 2.94 mM
2 CaCl2·2H2O (Sigma C-3881) 10 mL/L 1 g/400mL dH2O 0.17 mM
3 MgSO4·7H2O (Sigma 230391) 10 mL/L 3 g/400mL dH2O 0.3 mM
4 K2HPO4 (Sigma P 3786) 10 mL/L 3 g/400mL dH2O 0.43 mM
5 KH2PO4 (Sigma P 0662) 10 mL/L 7 g/400mL dH2O 1.29 mM
6 NaCl (Fisher S271-500) 10 mL/L 1 g/400mL dH2O 0.43 mM
7 P-IV Metal Solution 6 mL/L    
8 Vitamin B12 Solution 1 mL/L    
9 Biotin Vitamin Solution 1 mL/L    
10 Thiamine Vitamin Solution 1 mL/L