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Thiamine Vitamin Component Recipe

Directions

For 200 mL Total

  1. Prepare 200 mL of HEPES buffer (50 mM).
  2. Adjust the pH to 7.8.
  3. Add Thiamine (1 mM) wait until fully dissolved.
  4. Sterilize by 0.45 µm Millipore filter. Store in dark at freezer temperature.

**The amount of vitamins can vary from medium to medium so the final concentration is not listed.

# Component Amount Stock Solution Concentration Final Concentration
1 HEPES buffer pH 7.8
(Sigma H-3375)		 2.4 g/200 mL dH2O 50 mM **
2 Thiamine
(Sigma T-1270)		 0.067 g/200 mL dH2O ~ 1 mM **