10L Bristol Media Upgrade Kit: Bold 3N Medium
$ 25.00 $ 175.00
10L Bristol-to-Bold 3N Media Upgrade Kit
This upgrade kit includes all the necessary chemical and organic components needed to convert the 10L Bristol Media Kit into 10L of Bold 3N Medium. Bold 3N contains three times (3X) the amount of nitrogen as compared to Bristol, and includes the addition of a soilwater extract component, and vitamin B12.
Medium At A Glance |
|
| Water Type | Freshwater |
| pH of Medium | 6<pH<8 |
| Contains | NO3, Micronutrients, Vitamin B12 |
What is a media kit? How is it different than stock solutions?
UTEX Media Kits come with pre-measured, ready-to-use components. Unlike stock solutions, there is no need to prepare and/or dilute concentrated reagents for each individual chemical at precise ratios. Simply follow the included instructions to create the large volume of media—precision without the hassle of extra preparation.
Do the media kits include stock solutions?
No. UTEX Media Kits do not include concentrated stock solutions for each individual chemical component of a medium. Compatible chemicals have been pre-measured and combined to not only make preparation easier, but also reduce packaging and shipping costs.
What if I want individual stock solutions?
Each culture medium page includes a recipe that, when applicable, provides instructions for preparing stock solutions and how much to add. Because some components aren’t suitable for stock solutions, related instructions are provided only when applicable.
Looking for Bold 3N Medium in 1-Liter and test tube volumes?
Directions
To upgrade the 10L Bristol Media Kit to Bolds 3N Media:
- Obtain and wear the appropriate PPE.
- Prepare the Bristol Media from the 10L Media Kit as directed, except the final volume should be brought up to 9L (9,000 mL) only instead of 10L.
- Carefully add the solutions (components #1-3) in order from the Media Upgrade Kit to the 9L of Bristol Media.
- Bring the volume up to 10 Liters with d-H2O and mix well.
- If needed, autoclave or filter-sterilize the medium and allow to cool to room temperature.
- Store at refrigerator temperatures.
| Chemical | Final Concentration |
|---|---|
| NaNO3 | 8.82 mM |
| P-IV Metal Solution | View Recipe |
| Soilwater: GR+ Medium | View Recipe |
| Vitamin B12 | View Recipe |
Important Notes To Consider:
- The quality of water, including natural seawater, is important. The term 'dH2O' generally refers to distilled, deionized, distilled/deionized water, Milli-Q water (Millipore Corp.), etc. Natural seawater and natural freshwater should be obtained from a non-polluted source.
- When dissolving chemicals, wait for the first component to dissolve before adding the second. Stirring, and sometimes the addition of heat, is often required to dissolve the chemicals efficiently. Preparation of stock solutions for frequently made algal culture media make preparing media convenient but also necessary to avoid errors from weighing very tiny amounts.
- Attention should be given to the pH of the final medium. In most cases, if pH adjustment is required, this occurs before sterilization. Please note that autoclaving removes carbon dioxide from media lacking carbonate stabilizing buffers. This would make the medium very alkaline soon after removal from the autoclave. In these cases, you should wait approximately 24 hours for gaseous equilibrium before inoculating the medium.
