10L Media Kit: MES-Volvox Medium

General purpose medium for freshwater strains, especially those requiring ammonium. Suitable for xenic and axenic cultures. Modification of Volvox Medium. MES buffer is used in place of glycylglycine, and ammonium chloride is added as a second nitrogen source. 

Media kit includes all the necessary chemical components to prepare 10 Liters of MES-Volvox Medium. All UTEX Media Kit solutions are shipped sterile and should be handled aseptically to avoid contamination.

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Medium At A Glance

Water Type Freshwater
pH of Medium 6<pH<8
Contains Micronutrients, NH4, NO3, Biotin, Vitamin B12

Directions

For 10L Total Volume

  1. Obtain and wear the appropriate PPE.
  2. To approximately 9.7 Liters (9,700 mL) of dH2O, add the first three solutions (Components #1-3) in order while stirring.
    • Bring total volume to 10 Liters (10,000 mL) with dH2O.
    • Cover and autoclave or filter-sterilize if non-sterile water was used.
    • When cooled, aseptically add Component #4.
    • Store at refrigerator temperatures.

    Note: When combined this gives a pH of 6.5 before autoclaving. Both the standard medium and medium made from the 10L Media Kit show a pH of 6.5 - 6.6 after autoclaving.


    Components In Kit Final Concentration
    Ca(NO3)24H2O 0.5 mM
    MgSO47H2O 0.16 mM
    Na2glycerophosphate5H2O 0.16 mM
    KCl 0.67 mM
    NH4Cl 0.5 mM
    MES 10 mM
    P-IV Metal Solution
    Vitamin B12
    Biotin Vitamin Solution

    Important Notes To Consider:

    • The quality of water, including natural seawater, is important. The term 'dH2O' generally refers to distilled, deionized, distilled/deionized water, Milli-Q water (Millipore Corp.), etc. Natural seawater and natural freshwater should be obtained from a non-polluted source.
    • When dissolving chemicals, wait for the first component to dissolve before adding the second. Stirring, and sometimes the addition of heat, is often required to dissolve the chemicals efficiently. Preparation of stock solutions for frequently made algal culture media make preparing media convenient but also necessary to avoid errors from weighing very tiny amounts.
    • Attention should be given to the pH of the final medium. In most cases, if pH adjustment is required, this occurs before sterilization. Please note that autoclaving removes carbon dioxide from media lacking carbonate stabilizing buffers. This would make the medium very alkaline soon after removal from the autoclave. In these cases, you should wait approximately 24 hours for gaseous equilibrium before inoculating the medium.