$ 170.00 $ 550.00
General purpose medium for freshwater strains, especially those requiring ammonium. Suitable for xenic and axenic cultures. Modification of Volvox Medium. MES buffer is used in place of glycylglycine, and ammonium chloride is added as a second nitrogen source.
Media kit includes all the necessary chemical components to prepare 10 Liters of MES-Volvox Medium. All UTEX Media Kit solutions are shipped sterile and should be handled aseptically to avoid contamination.
Medium At A Glance |
|
Water Type | Freshwater |
pH of Medium | 6<pH<8 |
Contains | Micronutrients, NH4, NO3, Biotin, Vitamin B12 |
Note: When combined this gives a pH of 6.5 before autoclaving. Both the standard medium and medium made from the 10L Media Kit show a pH of 6.5 - 6.6 after autoclaving.
Components In Kit | Final Concentration |
---|---|
Ca(NO3)2•4H2O | 0.5 mM |
MgSO4•7H2O | 0.16 mM |
Na2glycerophosphate•5H2O | 0.16 mM |
KCl | 0.67 mM |
NH4Cl | 0.5 mM |
MES | 10 mM |
P-IV Metal Solution | |
Vitamin B12 | |
Biotin Vitamin Solution |