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WC+ Medium



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WC+ Medium

Component list is the same as WC Medium with the following exceptions:

  • The addition of 1 mL of DYV Metal Solution
  • Increase in the volume of Na2SiO3‡9H2O

Directions

For 1 L Total
  1. To approximately 900 mL of dd-H2O, add the following components in the orer listed (not including the vitamins) while stirring continuously.
  2. Adjust the pH to 7.8
  3. Transfer the contents of the beaker to a 1-Liter graduated cylinder and bring the total volume to 1.0 Liter with dd-H2O.
  4. Add the sterile vitamin components and mix well.
  5. Do not autoclave medium! Filter sterilize only.
  6. Store at refrigerator temperature.

# Component Amount Stock Solution Concentration Final Concentration
1 Na2SiO39H2O
(Sigma 307815)
2 mL/L 28.42 g/L 0.2 mM
2

NaNO3
(Fisher BP360-500)

1 mL/L 85.1 g/L 1 mM
3 CaCl22H2O
(Sigma C-3881)
1 mL/L 36.76 g/L 0.25 mM
4 MgSO47H2O
(Sigma 230391)
1 mL/L 36.97 g/L 0.15 mM
5 NaHCO3
(Fisher S 233)
1 mL/L 12.6 g/L 0.15 mM
6 K2HPO4
(Sigma P 3786)
1 mL/L 8.71 g/L 0.05 mM
7 H3BO3
(Baker 0084)
1 mL/L 24 g/L 0.39 mM
8 WC Trace Elements Solution 1 mL/L
9 DYV Metal Solution 1 mL/L
10 Vitamin B12 1 mL/L
11 Thiamine Vitamin Solution 1 mL/L
12 Biotin Vitamin Solution 1 mL/L

Important Notes To Consider:

  • The quality of water, including natural seawater, is important. The term 'dH2O' generally refers to distilled, deionized, distilled/deionized water, Milli-Q water (Millipore Corp.), etc. Natural seawater (and natural freshwater) should be obtained from a non-polluted source.
  • When dissolving chemicals, wait for the first component to dissolve before adding the second. Stirring, and sometimes the addition of heat, is often required to dissolve the chemicals efficiently. Preparation of stock solutions for frequently made algal culture media make preparing media convenient but also necessary to avoid errors from weighing very tiny amounts.
  • Attention should be given to the pH of the final medium. In most cases, if pH adjustment is required, this occurs before sterilization. Please note that autoclaving removes carbon dioxide from media lacking carbonate stabilizing buffers. This would make the medium very alkaline soon after removal from the autoclave. In these cases, you should wait approximately 24 hours for gaseous equilibrium before inoculating the medium.