WC Trace Elements Solution Recipe

To approximately 950 mL of dH₂O, first dissolve EDTA and then add the remaining components in order.
Transfer the contents of the beaker to a 1-Liter graduated cylinder and bring the total volume to 1 Liter with dH₂O.
Store in the refrigerator.

#	Component	Amount	Stock Solution Concentration	Final Concentration
1	Na₂EDTA•2H₂O (Sigma ED255)	4.36 g/L		11.7 µM
2	FeCl₃•6H₂O (Sigma F-1513)	3.15 g/L		11.7 µM
3	CuSO₄•5H₂O (MCIB 3M11)	1 mL/L	2.5 g/L	10 nM
4	ZnSO₄•7H₂O (Sigma Z 0251)	1 mL/L	22 g/L	76.5 nM
5	CoCl₂•6H₂O (Sigma C-3169)	1 mL/L	10 g/L	42 nM
6	MnCl₂•4H₂O (Baker 2540)	1 mL/L	180 g/L	910 nM
7	Na₂MoO₄•2H₂O (J.T. Baker 3764)	1 mL/L	6.3 g/L	26 nM
8	Na₃VO₄ (Sigma 450243)	1 mL/L	18 g/L	98 nM