WC Medium

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WC Medium Recipe

 

 

Directions

For 1 L Total

  1. To approximately 900 mL of dd-H2O, add the following components in the orer listed (not including the vitamins) while stirring continuously.
  2. Adjust the pH to 7.8
  3. Transfer the contents of the beaker to a 1-Liter graduated cylinder and bring the total volume to 1.0 Liter with dd-H2O.
  4. Add the sterile vitamin components and mix well.
  5. Do not autoclave medium! Filter sterilize only.
  6. Store at refrigerator temperature.

# Component Amount Stock Solution Concentration Final Concentration
1 NaNO3 (Fisher BP360-500) 1 mL/L 85.1 g/L 1 mM
2 CaCl2·2H2O (Sigma C-3881) 1 mL/L 36.76 g/L 0.25 mM
3 MgSO4·7H2O (Sigma 230391) 1 mL/L 36.97 g/L 0.15 mM
4 NaHCO3 (Fisher S 233) 1 mL/L 12.6 g/L 0.15 mM
5 Na2SiO3·9H2O (Sigma 307815) 1 mL/L 28.42 g/L 0.1 mM
6 K2HPO4 (Sigma P 3786) 1 mL/L 8.71 g/L 0.05 mM
7 H3BO3 (Baker 0084) 1 mL/L 24 g/L 0.39 mM
8 WC Trace Elements Solution 1 mL/L    
9 Vitamin B12 1 mL/L    
10 Thiamine Vitamin Solution 1 mL/L    
11 Biotin Vitamin Solution 1 mL/L