For 1 L Total
1. To approximately 900 mL of dd-H2O, add the following components in the orer listed (not including the vitamins) while stirring continuously.
2. Adjust the pH to 7.8.
3. Transfer the contents of the beaker to a 1-Liter graduated cylinder and bring the total volume to 1.0 Liter with dd-H2O.
4. Add the sterile vitamin components and mix well.
5. Do not autoclave medium! Filter sterilize only.
6. Store at refrigerator temperature.