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UTEX sells P49 Medium at 1X concentration only. Stock solutions and individual components for this specific medium are currently not available for purchase.

P49 Medium

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$ 55.00

Deadline to place an order for P49 Medium with a 2024 shipment date has passed. New orders will not ship until the 2025 new year—see announcement or order confirmation email for the expected ship date.

FAQs: Algal Culture Media

P49 Medium

Suitable for some volvocales, especially Chlamydomonas and Pandorina, which may benefit primarily from the ammonium component, modified volvox medium.

Directions

For 1 L Total
  1. To approximately 900 mL of dH2O, add each of the components in the order specified (except vitamins) while stirring continuously.
  2. Adjust the pH to 7.0
  3. Bring total volume to 1 L with dH2O.
    • For 1.5% agar medium add 15 grams of agar into the flask; do not mix.
  4. Cover and autoclave medium.
  5. When cooled add vitamins.
    • For agar medium add vitamins, mix, and dispense before agar solidifies.
  6. Store at refrigerator temperature.

# Component Amount Stock Solution Concentration Final Concentration
1 MgSO4•7H2O
(Sigma 230391)		 1 mL/L 4 g/100 mL dH2O 0.16 mM
2 Na2glycerophosphate•5H2O
(Sigma G 6501 )		 1 mL/L 5 g/100 mL dH2O 0.16 mM
3 KCl
(Fisher P 217)		 1 mL/L 5 g/100 mL dH2O 0.67 mM
4 Glycylglycine
(Sigma G 1002)		 10 mL/L 5 g/100 mL dH2O 3.8 mM
5 P-IV Metal Solution 6 mL/L
6 NH4NO3
(Fisher A676)		 10 mL/L 1 g/100 mL dH2O 1.2 mM
7 CaCl2•2H2O
(Sigma C-3881)		 10 mL/L 0.74 g/100 mL dH2O 0.5 mM
8 Yeast extract
(Bacto, Difco)		 0.2 g/L
9 Tryptone
(Sigma T 9410)		 0.4 g/L
10 Vitamin B12 1 mL/L
11 Biotin Vitamin Solution 1 mL/L
12 Thiamine Vitamin Solution 1 mL/L
P49 Medium Recipe PDF
Safety Data Sheet (SDS)
FAQs:Algal Culture Media

Important Notes To Consider:

  • The quality of water, including natural seawater, is important. The term 'dH2O' generally refers to distilled, deionized, distilled/deionized water, Milli-Q water (Millipore Corp.), etc. Natural seawater and natural freshwater should be obtained from a non-polluted source.
  • When dissolving chemicals, wait for the first component to dissolve before adding the second. Stirring, and sometimes the addition of heat, is often required to dissolve the chemicals efficiently. Preparation of stock solutions for frequently made algal culture media make preparing media convenient but also necessary to avoid errors from weighing very tiny amounts.
  • Attention should be given to the pH of the final medium. In most cases, if pH adjustment is required, this occurs before sterilization. Please note that autoclaving removes carbon dioxide from media lacking carbonate stabilizing buffers. This would make the medium very alkaline soon after removal from the autoclave. In these cases, you should wait approximately 24 hours for gaseous equilibrium before inoculating the medium.

Additional algal culture media recipes can be found at the following other major Collections' websites: