P49 Medium



P49 Medium Recipe

Suitable for some volvocales, especially Chlamydomonas and Pandorina, which may benefit primarily from the ammonium component, modified volvox medium.

 

Directions

For 1 L Total

  1. To approximately 900 mL of dH2O, add each of the components in the order specified (except vitamins) while stirring continuously.
  2. Adjust the pH to 7.0
  3. Bring total volume to 1 L with dH2O.
    • *For 1.5% agar medium add 15 grams of agar into the flask; do not mix.
  4. Cover and autoclave medium.
  5. When cooled add vitamins.
    • For agar medium add vitamins, mix, and dispense before agar solidifies.
  6. Store at refrigerator temperature.

# Component Amount Stock Solution Concentration Final Concentration
1 MgSO4·7H2O (Sigma 230391) 1 mL/L 4 g/100 mL dH2O 0.16 mM
2 Na2glycerophosphate.5H2O (Sigma G 6501 ) 1 mL/L 5 g/100 mL dH2O 0.16 mM
3 KCl (Fisher P 217) 1 mL/L 5 g/100 mL dH2O 0.67 mM
4 Glycylglycine (Sigma G 1002) 10 mL/L 5 g/100 mL dH2O 3.8 mM
5 P-IV Metal Solution 6 mL/L    
6 NH4NO3 (Fisher A676) 10 mL/L 1 g/100 mL dH2O 1.2 mM
7 CaCl2·2H2O (Sigma C-3881) 10 mL/L 0.74 g/100 mL dH2O 0.5 mM
8 Yeast extract (Bacto, Difco) 0.2 g/L    
9 Tryptone (Sigma T 9410) 0.4 g/L    
10 Vitamin B12 1 mL/L    
11 Biotin Vitamin Solution 1 mL/L    
12 Thiamine Vitamin Solution 1 mL/L