P49 Medium



P49 Medium Recipe

Directions 
Suitable for some volvocales, especially Chlamydomonas and Pandorina, which may benefit primarily from the ammonium component, modified volvox medium.

For 1 L Total

1. To approximately 900 mL of dH2O, add each of the components in the order specified (except vitamins) while stirring continuously.
2. Adjust the pH to 7.0.
3. Bring the total volume to 1 L with dH2O.
*For 1.5% agar medium add 15 g of agar into the flask; do not mix.
4. Cover and autoclave medium.
5. When cooled add vitamins.
*For agar medium add vitamins, mix, and dispense before agar solidifies.
6. Store at refrigerator temperature.

# Component Amount Stock Solution
Concentration
Final Concentration
1 MgSO4·7H2O (Sigma 230391) 1 mL/L 4 g/100 mL dH20 0.16 mM
2 Na2glycerophosphate.5H2O (Sigma G 6501 ) 1 mL/L 5 g/100 mL dH20 0.16 mM
3 KCl (Fisher P 217) 1 mL/L 5 g/100 mL dH20 0.67 mM
4 Glycylglycine (Sigma G 1002) 10 mL/L 5 g/100 mL dH20 3.8 mM
5 P-IV Metal Solution 6 mL/L    
6 NH4NO3 (Fisher A676) 10 mL/L 1 g/100 mL dH20 1.2 mM
7 CaCl2·2H2O (Sigma C-3881) 10 mL/L 0.74 g/100 mL dH20 0.5 mM
8 Yeast extract
(Bacto, Difco)
0.2 g/L    
9 Tryptone (Sigma T 9410)) 0.4 g/L    
10 Vitamin B12 1 mL/L    
11 Biotin Vitamin Solution 1 mL/L    
12 Thiamine Vitamin Solution 1 mL/L