UTEX sells Modified Artificial Seawater Medium at 1X concentration only. Stock solutions and individual components for this specific medium are currently not available for purchase.
Modified Artificial Seawater Medium
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$ 55.00
Deadline to place an order for Modified Artificial Seawater Medium with a 2024 shipment date has passed. New orders will not ship until the 2025 new year—see announcement or order confirmation email for the expected ship date.
Modified Artificial Seawater Medium
Modification of artificial seawater medium for B 2795 Picochlorum oklahomensis.
Directions
For 1 L Total
- To approximately 850 mL of dH2O, add each of the components in the order specified (except vitamins) while stirring continuously.
- Adjust the pH to 8.0.
- Bring the total volume to 1 L with dH2O.
- For 1.5% agar medium add 15 g of agar into the flask; do not mix.
- Cover and autoclave medium.
- When cooled add vitamins.
- For agar medium add vitamins, mix, and dispense before agar solidifies.
- Store at refrigerator temperature.
| # | Component | Amount | Stock Solution Concentration | Final Concentration |
|---|---|---|---|---|
| 1 | NaCl (Fisher S271-500) |
30 g/L | 0.51 M | |
| 2 | MgSO4•7H2O (Sigma 230391) |
10 mL/L | 24.4 g/100 mL dH20 | 9.9 mM |
| 3 | KCl (Fisher P 217) |
10 mL/L | 6 g/100 mL dH20 | 8 mM |
| 4 | NaNO3 (Fisher BP360-500) |
10 mL/L | 10 g/100 mL dH20 | 11.8 mM |
| 5 | CaCl2•2H2O (Sigma C-3881) |
10 mL/L | 3 g/100 mL dH20 | 2.04 mM |
| 6 | KH2PO4 (Sigma P 0662) |
10 mL/L | 0.5 g/100 mL dH20 | 0.37 mM |
| 7 | Tricine (Sigma T-5816-256) |
10 mL/L | 10 g/100 mL dH20 | 5.58 mM |
| 8 | NH4Cl (Fisher A 649-500) |
1 mL/L | 2.7 g/100 mL dH20 | 0.5 mM |
| 9 | P-IV Metal Solution | 1 mL/L | ||
| 10 | Soilwater: GR+ Medium | 30 mL/L | ||
| 11 | Vitamin B12 | 1 mL/L | ||
| 12 | Biotin Vitamin Solution | 1 mL/L | ||
| 13 | Thiamine Vitamin Solution | 1 mL/L |
Important Notes To Consider:
- The quality of water, including natural seawater, is important. The term 'dH2O' generally refers to distilled, deionized, distilled/deionized water, Milli-Q water (Millipore Corp.), etc. Natural seawater and natural freshwater should be obtained from a non-polluted source.
- When dissolving chemicals, wait for the first component to dissolve before adding the second. Stirring, and sometimes the addition of heat, is often required to dissolve the chemicals efficiently. Preparation of stock solutions for frequently made algal culture media make preparing media convenient but also necessary to avoid errors from weighing very tiny amounts.
- Attention should be given to the pH of the final medium. In most cases, if pH adjustment is required, this occurs before sterilization. Please note that autoclaving removes carbon dioxide from media lacking carbonate stabilizing buffers. This would make the medium very alkaline soon after removal from the autoclave. In these cases, you should wait approximately 24 hours for gaseous equilibrium before inoculating the medium.
Additional algal culture media recipes can be found at the following other major Collections' websites:
- Culture Collection of Algae and Protozoa (CCAP)
- National Center for Marine Algae and Microbiota (NCMA)
- National Institute for Environmental Studies (NIES) Microbial Culture Collection
- The Pasteur Culture Collection of Cyanobacteria (PCC)
- The Culture Collection of Algae at Goettingen University (SAG)
- Canadian Phycological Culture Centre (CPCC)
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