Modified Artificial Seawater Medium

UTEX sells Modified Artificial Seawater Medium at 1X concentration only. Stock solutions and individual components for this specific medium are currently not available for purchase.

Modified Artificial Seawater Medium

Saltwater Medium | 6 < pH < 8

Micronutrients NO3 Biotin Thiamine Vitamin B12

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$ 55.00

Modified Artificial Seawater Medium

Modification of artificial seawater medium for B 2795 Picochlorum oklahomensis.

Directions

For 1 L Total

To approximately 850 mL of dH₂O, add each of the components in the order specified (except vitamins) while stirring continuously.
Adjust the pH to 8.0.
Bring the total volume to 1 L with dH₂O.
- For 1.5% agar medium add 15 g of agar into the flask; do not mix.
Cover and autoclave medium.
When cooled add vitamins.
- For agar medium add vitamins, mix, and dispense before agar solidifies.
Store at refrigerator temperature.

#	Component	Amount	Stock Solution Concentration	Final Concentration
1	NaCl (Fisher S271-500)	30 g/L		0.51 M
2	MgSO₄•7H₂O (Sigma 230391)	10 mL/L	24.4 g/100 mL dH20	9.9 mM
3	KCl (Fisher P 217)	10 mL/L	6 g/100 mL dH20	8 mM
4	NaNO₃ (Fisher BP360-500)	10 mL/L	10 g/100 mL dH20	11.8 mM
5	CaCl₂•2H₂O (Sigma C-3881)	10 mL/L	3 g/100 mL dH20	2.04 mM
6	KH₂PO₄ (Sigma P 0662)	10 mL/L	0.5 g/100 mL dH20	0.37 mM
7	Tricine (Sigma T-5816-256)	10 mL/L	10 g/100 mL dH20	5.58 mM
8	NH₄Cl (Fisher A 649-500)	1 mL/L	2.7 g/100 mL dH20	0.5 mM
9	P-IV Metal Solution	1 mL/L
10	Soilwater: GR+ Medium	30 mL/L
11	Vitamin B₁₂	1 mL/L
12	Biotin Vitamin Solution	1 mL/L
13	Thiamine Vitamin Solution	1 mL/L

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Modified Artificial Seawater Medium Recipe PDF

Safety Data Sheet (SDS)

FAQs:Algal Culture Media

Important Notes To Consider:

The quality of water, including natural seawater, is important. The term 'dH₂O' generally refers to distilled, deionized, distilled/deionized water, Milli-Q water (Millipore Corp.), etc. Natural seawater and natural freshwater should be obtained from a non-polluted source.
When dissolving chemicals, wait for the first component to dissolve before adding the second. Stirring, and sometimes the addition of heat, is often required to dissolve the chemicals efficiently. Preparation of stock solutions for frequently made algal culture media make preparing media convenient but also necessary to avoid errors from weighing very tiny amounts.
Attention should be given to the pH of the final medium. In most cases, if pH adjustment is required, this occurs before sterilization. Please note that autoclaving removes carbon dioxide from media lacking carbonate stabilizing buffers. This would make the medium very alkaline soon after removal from the autoclave. In these cases, you should wait approximately 24 hours for gaseous equilibrium before inoculating the medium.