Modified Artificial Seawater Medium

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Modified Artificial Seawater Medium Recipe

Modification of Artificial Seawater Medium for B 2795 Picochlorum oklahomensis.

 

Directions

For 1 L Total

  1. To approximately 850 mL of dH2O, add each of the components in the order specified (except vitamins) while stirring continuously.
  2. Adjust the pH to 8.0
  3. Bring the total volume to 1 L with dH2O.
    • *For 1.5% agar medium add 15 grams of agar into the flask; do not mix.
  4. Cover and autoclave medium.
  5. When cooled add vitamins
    • *For agar medium add vitamins, mix, and dispense before agar solidifies.
  6. Store at refrigerator temperature.

# Component Amount Stock Solution Concentration Final Concentration
1 NaCl (Fisher S271-500) 30 g/L   0.51 M
2 MgSO4·7H2O (Sigma 230391) 10 mL/L 24.4 g/100 mL dH2O 9.9 mM
3 KCl (Fisher P 217) 10 mL/L 6 g/100 mL dH2O 8 mM
4 NaNO3 (Fisher BP360-500) 10 mL/L 10 g/100 mL dH2O 11.8 mM
5 CaCl2·2H2O (Sigma C-3881) 10 mL/L 3 g/100 mL dH2O 2.04 mM
6 KH2PO4 (Sigma P 0662) 10 mL/L 0.5 g/100 mL dH2O 0.37 mM
7 Tricine (Sigma T-5816-256) 10 mL/L 10 g/100 mL dH2O 5.58 mM
8 NH4Cl (Fisher A 649-500) 1 mL/L 2.7 g/100 mL dH2O 0.5 mM
9 P-IV Metal Solution 1 mL/L    
10 Soilwater: GR+ Medium 30 mL/L    
11 Vitamin B12 1 mL/L    
12 Biotin Vitamin Solution 1 mL/L    
13 Thiamine Vitamin Solution 1 mL/L