F/2 Medium

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Orders for algal culture media placed after 5:00 PM CST on Wednesday, November 14th through Wednesday, November 28th, will not ship until Monday, December 3rd, 2018. There will be no outgoing shipments on November 26th, 2018. Please view more information here.

F/2 Medium Recipe


For 1 L Total

  1. To approximately 950 mL of non-pasteurized seawater ( 30-35ppt), add each of the components in the order specified (except vitamins) while stirring continuously.
  2. Bring total volume to 1 L with non-pasturized seawater.
    • *For 1.5% (w/v) agar medium: add 15 g of agar into the flask; do not mix.
  3. Cover and autoclave medium. 
  4. Add sterile vitamins to cooled medium.
    • *For agar medium add vitamins, mix, and dispense before agar solidifies. 
  5. Store at refrigerator temperature.

# Component Amount Stock Solution Concentration Final Concentration
1 NaNO3 (Fisher BP360-500) 1 mL/L 7.5 g/100 mL dH2O 880 µM
2 NaH2PO4·H2O(MCIB 742) 1 mL/L 0.5 g/100 mL dH2O 36 µM
3 Na2SiO3·9H2O (Sigma 307815) 1 mL/L 2 g/100 mL dH2O 70 µM
6 Trace Metals Solution 1 mL/L    
7 Vitamin B12 Solution 1 mL/L    
8 Biotin Vitamin Solution 1 mL/L    
9 Thiamine Vitamin Solution 1 mL/L