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Trace Metals Solution Recipe

Directions

For 1 L Total

  1. Begin stirring and heating approximately 950 mL of dH2O.
  2. When the stirring water approaches the boiling point, add the following components in the order listed.
  3. Bring the volume to 1 L with dH2O
  4. Store at refrigerator temperature.
# Component Amount Stock Solution Concentration Final Concentration
1 ZnSO4•7H2O
(Sigma Z 0251)		 23 mg/L 0.08 µM
2 MnSO4•H2O
(Sigma M8179)		 152 mg/L 0.9 µM
3 Na2MoO4•2H2O
(J.T. Baker 3764)		 7.3 mg/L 0.03 µM
4 CoSO4•7H2O
(Baker 1696)		 14 mg/L 0.05 µM
5 CuCl2•2H2O
(Fisher C-455)		 6.8 mg/L 0.04 µM
6 Fe(NH4)2(SO4)2•6H2O
(Sigma F-1513)		 4.6 g/L 11.7 µM
7 Na2EDTA•2H2O
(Sigma ED255)		 4.4 g/L 11.7 µM

Note: Final concentration listed is for the stock solution.