Trace Metals Solution Recipe
Directions
For 1 L Total
- Begin stirring and heating approximately 950 mL of dH2O.
- When the stirring water approaches the boiling point, add the following components in the order listed.
- Bring the volume to 1 L with dH2O
- Store at refrigerator temperature.
| # | Component | Amount | Stock Solution Concentration | Final Concentration |
|---|---|---|---|---|
| 1 | ZnSO4•7H2O (Sigma Z 0251) |
23 mg/L | 0.08 µM | |
| 2 | MnSO4•H2O (Sigma M8179) |
152 mg/L | 0.9 µM | |
| 3 | Na2MoO4•2H2O (J.T. Baker 3764) |
7.3 mg/L | 0.03 µM | |
| 4 | CoSO4•7H2O (Baker 1696) |
14 mg/L | 0.05 µM | |
| 5 | CuCl2•2H2O (Fisher C-455) |
6.8 mg/L | 0.04 µM | |
| 6 | Fe(NH4)2(SO4)2•6H2O (Sigma F-1513) |
4.6 g/L | 11.7 µM | |
| 7 | Na2EDTA•2H2O (Sigma ED255) |
4.4 g/L | 11.7 µM |
Note: Final concentration listed is for the stock solution.
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