Enrichment Solution for Seawater Medium Recipe
Directions
For 2 L Total
- To approximately 1 L of dH2O, add each of the components in the order specified (except vitamins) while stirring continuously.
- Adjust the pH to 7.8.
- Bring the total volume to 2 Liters with dH2O.
| # | Component | Amount | Stock Solution Concentration | Final Concentration |
|---|---|---|---|---|
| 1 | NaNO3 (Fisher BP360) |
4.7 g/2 L | 27.65 mM | |
| 2 | Na2glycerophosphate•5H2O (Sigma G 6501 ) |
0.7 g/2 L | 1.6 mM | |
| 3 | ES Fe Solution | 325 mL/2 L | ||
| 4 | P-II Metal Solution | 325 mL/2 L | ||
| 5 | HEPES buffer (Sigma H 3375) |
6.5 g/2 L | 14 mM | |
| 6 | Vitamin B12 | 3 mL/2 L | ||
| 7 | Biotin Vitamin Solution | 3 mL/2 L | ||
| 8 | Thiamine Vitamin Solution | 3 mL/2 L |
Note: Final concentration listed is for the stock solution.
Follow UTEX
All UTEX images are licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.
