Enrichment Solution for Seawater Medium Recipe
Directions
For 2 L Total
- To approximately 1 L of dH2O, add each of the components in the order specified (except vitamins) while stirring continuously.
- Adjust the pH to 7.8.
- Bring the total volume to 2 Liters with dH2O.
| # | Component | Amount | Stock Solution Concentration | Final Concentration |
|---|---|---|---|---|
| 1 | NaNO3 (CAS: 7631-99-4) |
4.7 g/2 L | 27.65 mM | |
| 2 | Na2glycerophosphate•5H2O (CAS: 13408-09-8) |
0.98 g/2 L | 1.6 mM | |
| 3 | ES Fe Solution | 325 mL/2 L | ||
| 4 | P-II Metal Solution | 325 mL/2 L | ||
| 5 | HEPES buffer (CAS: 7365-45-9) |
6.5 g/2 L | 14 mM | |
| 6 | Vitamin B12 | 3 mL/2 L | ||
| 7 | Biotin Vitamin Solution | 3 mL/2 L | ||
| 8 | Thiamine Vitamin Solution | 3 mL/2 L |
Note: Final concentration listed is for the stock solution.
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