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P-II Metal Solution Recipe

Directions

For 100 mL Total

  1. To approximately 70 mL of dH2O, add each of the components in the order specified while stirring continuously.
  2. Bring total volume to 100 mL with dH2O.
  3. Store at refrigerator temperature.
    • Note: CoCL2 can be interchancable with CoSO4.
# Component Amount Stock Solution Concentration Final Concentration
1 Na2EDTA•2H2O
(Sigma ED255)		 0.1 g/100 mL 2.7 mM
2 H3BO3
(Baker 0084)		 0.114 g/100 mL 18.4 mM
3 FeCl3•6H2O
(Sigma F-1513)		 4.9 mg/100 mL 0.18 mM
4 MnSO4•H2O
(Sigma M8179)		 16.4 mg/100 mL 0.97 mM
5 ZnSO4•7H2O
(Sigma Z 0251)		 2.2 mg/100 mL 0.07 mM
6 CoCl2•6H2O
(Sigma C-3169)		 0.48 mg/100 mL 0.02 mM

Note: Final concentration listed is for the stock solution.