Dasycladales Seawater Medium



Dasycladales Seawater Medium

Directions 

For 3 L Total


1. Prepare 3 L of Pasteurized Seawater (30 ppt).
2. Add the remaining sterile media solutions to the flask in the order listed.
3. Store at refrigerator temperature.


# Component Amount Stock Solution
Concentration
Final Concentration
1 Pasteurized Seawater 3 L    
2 Soil+Seawater Medium 50 mL/3 L    
3 DAS Macro Solution 40 mL/3 L    
4 Modified P-IV chelated Micronutrient Solution 30 mL/3 L    
5 DAS Vitamin Cocktail 1.5 mL/3 L