Dasycladales Seawater Medium

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Dasycladales Seawater Medium Recipe

 

 

Directions

For 3 L Total

  1. Prepare 3 L of Pasteurized Seawater (30 ppt).
  2. Add the remaining sterile media solutions to the flask in the order listed.
  3. Store at refrigerator temperature.

# Component Amount Stock Solution Concentration Final Concentration
1 Pasteurized Seawater 3 L    
2 Soil+Seawater Medium 50 mL/3 L    
3 DAS Macro Solution 40 mL/3 L    
4 Modified P-IV chelated Micronutrient Solution 30 mL/3 L    
5 DAS Vitamin Cocktail 1.5 mL/3 L