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DAS Macro Solution Recipe

Directions

For 40 mL total

  1. Aseptically add the following components in the order listed.
  2. Swirl gently to mix contents.
  3. Store at refrigerator temperature.
# Component Amount Stock Solution Concentration Final Concentration in DAS
1 NaNO3
*autoclave before adding*
(Fisher BP360)		 10 mL/3 L 0.70 M 2.3 mM
2 Na2HPO4•7H2O
*autoclave before adding*
(Sigma S 9390)		 30 mL/3 L 0.02 M 0.2 mM