DAS Macro Solution Recipe
Directions
For 40 mL total
- Aseptically add the following components in the order listed.
- Swirl gently to mix contents.
- Store at refrigerator temperature.
| # | Component | Amount | Stock Solution Concentration | Final Concentration in DAS |
|---|---|---|---|---|
| 1 | NaNO3 *autoclave before adding* (Fisher BP360) |
10 mL/3 L | 0.70 M | 2.3 mM |
| 2 | Na2HPO4•7H2O *autoclave before adding* (Sigma S 9390) |
30 mL/3 L | 0.02 M | 0.2 mM |
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