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Modified P-IV Chelated Micronutrient Solution Component Recipe

Directions

For 1 L Total

  1. To approximately 900 mL of dH2O, add the following components in the order listed while stirring continuously.
    • Note: Wait until the chelators (the first two components) have dissolved fully before adding the remainin
  2. Bring total volume to 1 L with dH2O.
  3. Autoclave to sterilize.
  4. Store at refrigerator temperature.
# Component Amount Stock Solution Concentration Final Concentration in Medium
1 Na2EDTA•2H2O
(Sigma ED255)		 0.2 g/L 0.54 mM 5.4 µM
2 HEEDTA
(Sigma H-7154)		 0.03 g/L 0.11 mM 1.1 µM
3 FeCl3•6H2O
(Sigma F-1513)		 0.1 g/L 0.37 mM 3.7 µM
4 MnCl2•4H2O
(Baker 2540)		 0.04 g/L 0.20 mM 2.02 µM
5 ZnCl2
(Sigma Z-0152)		 0.005 g/L 0.037 mM 0.37 µM
6 CoCl2•6H2O
(Sigma C-3169)		 0.002 g/L 0.008 mM 0.084 µM
7 Na2MoO4•2H2O
(J.T. Baker 3764)		 0.004 g/L 0.017 mM 0.17 µM