Modified P-IV Chelated Micronutrient Solution Component Recipe

To approximately 900 mL of dH₂O, add the following components in the order listed while stirring continuously.
- Note: Wait until the chelators (the first two components) have dissolved fully before adding the remainin
Bring total volume to 1 L with dH₂O.
Autoclave to sterilize.
Store at refrigerator temperature.

#	Component	Amount	Stock Solution Concentration	Final Concentration in Medium
1	Na₂EDTA•2H₂O (Sigma ED255)	0.2 g/L	0.54 mM	5.4 µM
2	HEEDTA (Sigma H-7154)	0.03 g/L	0.11 mM	1.1 µM
3	FeCl₃•6H₂O (Sigma F-1513)	0.1 g/L	0.37 mM	3.7 µM
4	MnCl₂•4H₂O (Baker 2540)	0.04 g/L	0.20 mM	2.02 µM
5	ZnCl₂ (Sigma Z-0152)	0.005 g/L	0.037 mM	0.37 µM
6	CoCl₂•6H₂O (Sigma C-3169)	0.002 g/L	0.008 mM	0.084 µM
7	Na₂MoO₄•2H₂O (J.T. Baker 3764)	0.004 g/L	0.017 mM	0.17 µM