Modified P-IV Chelated Micronutrient Solution Component Recipe


Modified P-IV Chelated Micronutrient Solution Component Recipe

Directions

For 1 L Total

  1. To approximately 900 mL of dH2O, add the following components in the order listed while stirring continuously.
    • Note: Wait until the chelators (the first 2 components) have dissolved fully before adding the remaining components to the stirring beaker.
  2. Bring total volume to 1 L with dH2O.
  3. Autoclave to sterilize.
  4. Store at refrigerator temperature.

Note final concentration listed is for the stock solution.


# Component Amount Stock Solution Concentration Final Concentration
1 Na2EDTA·2H2O (Sigma ED255) 0.2 g 0.54 mM
2 HEEDTA (Sigma H-7154) 0.03 g 0.11 mM
3 FeCl3·6H2O (Sigma F-1513) 0.1 g 0.37 mM
4 MnCl2·4H2O (Baker 2540) 0.04 g 0.20 mM
5 ZnCl2 (Sigma Z-0152) 0.005 g 0.037 mM
6 CoCl2·6H2O (Sigma C-3169) 0.002 g 0.008 mM
7 Na2MoO4·2H2O (J.T. Baker 3764) 0.004 g 0.017 mM

 

UTEX only sells complete media at 1X concentration. Stock solutions and individual components are currently not available for purchase.