Soil + Seawater Medium



Soil + Seawater Medium Recipe

For marine algae that require salinities at 30 ppt.

Directions

For 200 mL Total

  1. Combine components.
  2. Cover the medium container and steam for 2 consecutive days, 3 hours on each day. Pasteurization is a gradual rising of temperature to approximately 95 °C in 15 minutes. Then increased just over 98 °C for the 3 hour duration. Cooling occurs gradually at room temperature.
  3. Refrigerate 24 hours or more and bring to room temperature before use.

# Component Amount Stock Solution Concentration Final Concentration
1 Green House Soil 1 tsp
2 CaCO3 (optional) (Fisher C 64) 1 mg 0.05 mM
3 Pasteurized Seawater 200 mL