8 ppt F/2 Medium
Directions
For 1 L Total
- Dilute 1 Liter of seawater to 8 ppt.
- To approximately 950 mL of the non-pasteurized seawater (8 ppt), add each of the components in the order specified (except vitamins) while stirring continuously.
- Bring total volume to 1 L with remaining non-pasteurized seawater (8 ppt).
- For 1.5% agar medium add 15 g of agar into the flask; do not mix.
- Cover and autoclave medium.
- When cooled add sterile vitamins.
- For agar medium add vitamins, mix, and dispense before agar solidifies.
- Store at refrigerator temperature.
| # | Component | Amount | Stock Solution Concentration | Final Concentration |
|---|---|---|---|---|
| 1 | NaNO3 (Fisher BP360-500) |
1 mL | 7.5 g/100 mL dH20 | 880 µM |
| 2 | NaH2PO4•H2O (MCIB 742) |
1 mL | 0.5 g/100 mL dH20 | 36 µM |
| 3 | Na2SiO3•9H2O (Sigma 307815) |
1 mL | 3 g/100 mL dH20 | 106 µM |
| 4 | Trace Metals Solution | 1 mL/L | ||
| 5 | Vitamin B12 | 1 mL/L | ||
| 6 | Biotin Vitamin Solution | 1 mL/L | ||
| 7 | Thiamine Vitamin Solution | 1 mL/L |
Important Notes To Consider:
- The quality of water, including natural seawater, is important. The term 'dH2O' generally refers to distilled, deionized, distilled/deionized water, Milli-Q water (Millipore Corp.), etc. Natural seawater and natural freshwater should be obtained from a non-polluted source.
- When dissolving chemicals, wait for the first component to dissolve before adding the second. Stirring, and sometimes the addition of heat, is often required to dissolve the chemicals efficiently. Preparation of stock solutions for frequently made algal culture media make preparing media convenient but also necessary to avoid errors from weighing very tiny amounts.
- Attention should be given to the pH of the final medium. In most cases, if pH adjustment is required, this occurs before sterilization. Please note that autoclaving removes carbon dioxide from media lacking carbonate stabilizing buffers. This would make the medium very alkaline soon after removal from the autoclave. In these cases, you should wait approximately 24 hours for gaseous equilibrium before inoculating the medium.
Additional algal culture media recipes can be found at the following other major Collections' websites:
- Culture Collection of Algae and Protozoa (CCAP)
- National Center for Marine Algae and Microbiota (NCMA)
- National Institute for Environmental Studies (NIES) Microbial Culture Collection
- The Pasteur Culture Collection of Cyanobacteria (PCC)
- The Culture Collection of Algae at Goettingen University (SAG)
- Canadian Phycological Culture Centre (CPCC)
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