For marine algae that require salinities at 48 ppt.
# | Component | Amount | Stock Solution Concentration | Final Concentration |
---|---|---|---|---|
1 | Seawater (non-sterilized) |
1 L | ||
2 | Natural sea-salt | 15 g/L | ||
3 | NaNO3 (Fisher BP360-500) |
1 mL | 7.5 g/100 mL dH20 | 880 µM |
4 | NaH2PO4•H2O (MCIB 742) |
1 mL | 0.5 g/100 mL dH20 | 36 µM |
5 | Na2SiO3•9H2O (Sigma 307815) |
1 mL | 2 g/100 mL dH20 | 70 µM |
6 | Trace Metals Solution | 1 mL/L | ||
7 | Vitamin B12 | 1 mL/L | ||
8 | Biotin Vitamin Solution | 1 mL/L | ||
9 | Thiamine Vitamin Solution | 1 mL/L |