Volvox-Dextrose Medium

UTEX sells Volvox-Dextrose Medium at 1X concentration only. Stock solutions and individual components for this specific medium are currently not available for purchase.

Volvox-Dextrose Medium

Freshwater Medium | 6 < pH < 8

Micronutrients NO3 Organic Supplements Biotin Vitamin B12

View Media Recipe

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$ 55.00

Deadline to place an order for Volvox-Dextrose Medium with a 2024 shipment date:
Dec 11, 2024 at 5:00 PM CST

Volvox-Dextrose Medium

Directions

For 1 L Total

Prepare 1 L of Volvox Medium, except add 10 grams of glucose after all other stock solutions have been added.
Bring total volume to 1 L with dH₂O.
- For 1.5% agar medium add 15 grams of agar into the flask; do not mix.
Cover and autoclave medium.
When cooled add vitamins.
- For agar medium add vitamins, mix, and dispense before agar solidifies.

#	Component	Amount	Final Concentration
1	Volvox Medium	1 L
2	Glucose (BACTO-dextrose or Sigma D 9634)	10 g/L	0.06 M
3	Vitamin B₁₂	1 mL/L
4	Biotin Vitamin Solution	1 mL/L

Volvox-Dextrose Medium Recipe PDF

Safety Data Sheet (SDS)

FAQs:Algal Culture Media

Important Notes To Consider:

The quality of water, including natural seawater, is important. The term 'dH₂O' generally refers to distilled, deionized, distilled/deionized water, Milli-Q water (Millipore Corp.), etc. Natural seawater and natural freshwater should be obtained from a non-polluted source.
When dissolving chemicals, wait for the first component to dissolve before adding the second. Stirring, and sometimes the addition of heat, is often required to dissolve the chemicals efficiently. Preparation of stock solutions for frequently made algal culture media make preparing media convenient but also necessary to avoid errors from weighing very tiny amounts.
Attention should be given to the pH of the final medium. In most cases, if pH adjustment is required, this occurs before sterilization. Please note that autoclaving removes carbon dioxide from media lacking carbonate stabilizing buffers. This would make the medium very alkaline soon after removal from the autoclave. In these cases, you should wait approximately 24 hours for gaseous equilibrium before inoculating the medium.