Volvox-Dextrose Medium



Volvox-Dextrose Medium Recipe

 

 

Directions

For 1 L Total

  1. Prepare 1 L of Volvox Medium, except add 10 grams of glucose after all other stock solutions have been added.
  2. Bring total volume to 1 L with dH2O.
    • *For 1.5% agar medium add 15 grams of agar into the flask; do not mix.
  3. Cover and autoclave medium.
  4. When cooled add vitamins.
    • *For agar medium add vitamins, mix, and dispense before agar solidifies.

# Component Amount Stock Solution Concentration Final Concentration
1 Volvox Medium 1 L    
2 Glucose (BACTO-dextrose or Sigma D 9634) 10 g/L   0.06 M
3 Vitamin B12 1 mL/L    
4 Biotin Vitamin Solution 1 mL/L