Suitable for axenic cultures of some colonial volvocales.
For 1 L Total
1. Prepare 1 L of Waris Medium, but do not adjust the pH. 2. Prepare 200 mL of Euglena Medium. 3. Discard 200 mL of the prepared Waris Medium. Transfer the remaining 800 mL to a 2 L Erlenmeyer flask. 4. Transfer 200 mL of the previously prepared Euglena Medium into the flask. * For agar add 10.0 g of agar into the flask; do not mix. 5. Cover and autoclave. 6. Store at refrigerator temperature.