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Volvocacean Medium



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Volvocacean Medium

Suitable for axenic cultures of some colonial volvocales.

Directions

For 1 L Total
  1. Prepare 1 L of Waris Medium, but do not adjust the pH.
  2. Prepare 200 mL of Euglena Medium.
  3. Discard 200 mL of the prepared Waris Medium. Transfer the remaining 800 mL to a 2 L Erlenmeyer flask.
  4. Transfer 200 mL of the previously prepared Euglena Medium into the flask.
    • For agar add 10.0 grams of agar into the flask; do not mix.
  5. Cover and autoclave.
  6. Store at refrigerator temperature.

# Component Amount Stock Solution Concentration Final Concentration
1 Waris Medium 800 mL
2 Euglena Medium 200 mL

Important Notes To Consider:

  • The quality of water, including natural seawater, is important. The term 'dH2O' generally refers to distilled, deionized, distilled/deionized water, Milli-Q water (Millipore Corp.), etc. Natural seawater (and natural freshwater) should be obtained from a non-polluted source.
  • When dissolving chemicals, wait for the first component to dissolve before adding the second. Stirring, and sometimes the addition of heat, is often required to dissolve the chemicals efficiently. Preparation of stock solutions for frequently made algal culture media make preparing media convenient but also necessary to avoid errors from weighing very tiny amounts.
  • Attention should be given to the pH of the final medium. In most cases, if pH adjustment is required, this occurs before sterilization. Please note that autoclaving removes carbon dioxide from media lacking carbonate stabilizing buffers. This would make the medium very alkaline soon after removal from the autoclave. In these cases, you should wait approximately 24 hours for gaseous equilibrium before inoculating the medium.