Euglena Medium

Euglena Medium Recipe

Suitable for most axenic cultures of Euglena and Chlorogonium.

For 1 L Total

1. To approximately 950 mL of dH2O, add each of the components in the order specified while stirring continuously.
2. Bring the total volume to 1 L with dH2O.
*For 1.5% agar medium add 15 g of agar into the flask; do not mix.
3. Cover and autoclave medium.
4. Store at refrigerator temperature.

# Component Amount Stock Solution
Final Concentration
1 Sodium acetate (Fisher BP 333) 1 g/L   12.2 mM
2 Beef extract
(Sigma B-4888)
1 g/L    
3 Tryptone (Sigma T 9410)) 2 g/L    
4 Yeast extract
(Bacto, Difco)
2 g/L    
5 CaCl2·2H2O (Sigma C-3881) 0.01 g/L   0.07 mM