Waris Medium



Waris Medium Recipe

Modification of the original recipe. Suitable for xenic cultures of some desmids.

 

Directions

For 1 L Total

  1. To approximately 900 mL of dH2O, add each of the components in the order specified while stirring continuously.
  2. Adjust the pH to 6.0.
  3. Bring the total volume to 1 L with dH2O.
  4. Cover and autoclave medium.

# Component Amount Stock Solution Concentration Final Concentration
1 KNO3 (Baker 3190) 1 mL/L 10 g/100 mL dH2O 0.99 mM
2 MgSO4·7H2O (Sigma 230391) 1 mL/L 2 g/100 mL dH2O 0.081 mM
3 (NH4)2HPO4 (Fisher A686) 1 mL/L 2 g/100 mL dH2O 0.151 mM
4 CaSO4.2H2O (Mallinckroft 4300) 30 mL/L 0.2 g/100 mL dH2O 0.35 mM
5 P-IV Metal Solution 6 mL/L