Waris Medium



Waris Medium Recipe

Directions 
Modification of the original recipe. Suitable for xenic cultures of some desmids.

For 1 L Total

1. To approximately 900 mL of dH2O, add each of the components in the order specified while stirring continuously.
2. Adjust the pH to 6.0.
3. Bring the total volume to 1 L with dH2O.
4. Cover and autoclave medium.

# Component Amount Stock Solution
Concentration
Final Concentration
1 KNO3 (Baker 3190) 1 mL/L 10 g/100 mL dH20 0.99 mM
2 MgSO4·7H2O (Sigma 230391) 1 mL/L 2 g/100 mL dH20 0.081 mM
3 (NH4)2HPO4 (Fisher A686) 1 mL/L 2 g/100 mL dH20 0.151 mM
4 CaSO4.2H2O (Mallinckroft 4300) 30 mL/L 0.2 g/100 mL dH20 0.35 mM
5 P-IV Metal Solution 6 mL/L