UTEX sells Volvocacean-3N Medium at 1X concentration only. Stock solutions and individual components for this specific medium are currently not available for purchase.

Volvocacean-3N Medium

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Volvocacean-3N Medium

R.C. Starr's modification of Volvocacean medium (E.G. Pringsheim, pers. Comm.), i.e., the amount of (NH4)2HPO4 is increased 3-fold, for axenic cultures of Eudorina (1204, 1205 and 1206) and other colonial volvocales.


For 1 L Total
  1. To approximately 900 mL of dH2O, add the following components in the order listed except Euglena Medium while stirring continuously.
  2. Adjust the pH to 6.0
  3. Bring total volume to 1 L with dH2O.
  4. Discard 200 mL of this medium.
  5. Transfer 200 mL of the previously prepared Euglena medium into the flask.
    • For agar add 10.0 grams of agar into the flask; do not mix.
  6. Autoclave medium.
  7. Store at refrigerator temperature.

# Component Amount Stock Solution Concentration Final Concentration
1 KNO3
(Baker 3190)
1 mL/L 10 g/100 mL dH2O 0.98 mM
2 MgSO47H2O
(Sigma 230391)
1 mL/L 2 g/100 mL dH2O 0.081 mM
3 (NH4)2HPO4
(Fisher A686)
3 mL/L 2 g/100 mL dH2O 0.453 mM
4 CaSO42H2O
(Mallinckroft 4300)
30 mL/L 0.2 g/100 mL dH2O 0.35 mM
5 P-IV Metal Solution 6 mL/L
6 Euglena Medium 200 mL/L

Important Notes To Consider:

  • The quality of water, including natural seawater, is important. The term 'dH2O' generally refers to distilled, deionized, distilled/deionized water, Milli-Q water (Millipore Corp.), etc. Natural seawater and natural freshwater should be obtained from a non-polluted source.
  • When dissolving chemicals, wait for the first component to dissolve before adding the second. Stirring, and sometimes the addition of heat, is often required to dissolve the chemicals efficiently. Preparation of stock solutions for frequently made algal culture media make preparing media convenient but also necessary to avoid errors from weighing very tiny amounts.
  • Attention should be given to the pH of the final medium. In most cases, if pH adjustment is required, this occurs before sterilization. Please note that autoclaving removes carbon dioxide from media lacking carbonate stabilizing buffers. This would make the medium very alkaline soon after removal from the autoclave. In these cases, you should wait approximately 24 hours for gaseous equilibrium before inoculating the medium.