Volvocacean-3N Medium



Volvocacean-3N Medium Recipe

Directions 
R.C. Starr's modification of Volvocacean medium (E.G. Pringsheim, pers. Comm.), i.e., the amount of (NH4)2HPO4 is increased 3-fold, for axenic cultures of Eudorina (1204, 1205 and 1206) and other colonial volvocales.

For 1 L Total

1. To approximately 900 mL of dH2O, add the following components in the order listed except Euglena medium while stirring continuously.
2. Adjust the pH to 6.0
3. Bring total volume to 1 L with dH2O.
4. Discard 200 mL of this medium.
5. Transfer 200 mL of Euglena Medium into the flask.
* For agar pour 10 g of agar into the flask; do not mix.
6. Autoclave medium.
7. Store at refrigerator temperature.

# Component Amount Stock Solution
Concentration
Final Concentration
1 KNO3 (Baker 3190) 1 mL/L 10 g/100 mL dH20 0.98 mM
2 MgSO4·7H2O (Sigma 230391) 1 mL/L 2 g/100 mL dH20 0.081 mM
3 (NH4)2HPO4 (Fisher A686) 3 mL/L 2 g/100 mL dH20 0.453 mM
4 CaSO4.2H2O (Mallinckroft 4300) 30 mL/L 0.2 g/100 mL dH20 0.35 mM
5 P-IV Metal Solution 6 mL/L    
6 Euglena Medium 200 mL/L