LDM Medium



LDM Medium Recipe

Modification of Lewin's marine diatom medium.

Directions

For 1 L Total

  1. To approximately 950 mL of pasteurized seawater (30 ppt), add each of the components in the order specified (except vitamins) while stirring continuously.
  2. Bring total volume to 1 L with non-pasteurized seawater.
    • *For 1.5% (w/v) agar medium, add 12 g of agar into the flaskl; do not mix.
  3. Cover and autoclave medium.
  4. When cooled add sterile vitamins.
    • *For agar medium add vitamin, mix, and dispense before agar solidifies.
  5. Store at refrigerator temperature.

# Component Amount Stock Solution Concentration Final Concentration
1 Pasteurized Seawater 950 mL
2 NaNO3 (Fisher BP360-500) 1 mL/L 10 g/400mL dH2O 0.29 mM
3 CaCl2·2H2O (Sigma C-3881) 1 mL/L 1 g/400mL dH2O 0.017 mM
4 MgSO4·7H2O (Sigma 230391) 1 mL/L 3 g/400mL dH2O 0.03 mM
5 K2HPO4 (Sigma P 3786) 1 mL/L 3 g/400mL dH2O 0.043 mM
6 KH2PO4 (Sigma P 0662) 1 mL/L 7 g/400mL dH2O 0.129 mM
7 NaCl (Fisher S271-500) 1 mL/L 1 g/400mL dH2O 0.043 mM
8 P-IV Metal Solution 6 mL/L    
9 Tryptone (Sigma T 9410) 1 g/L    
10 Vitamin B12 Solution 1 mL/L    
11 Biotin Vitamin Solution 1 mL/L