J Medium



J Medium Recipe

Directions 
Modification of Parker's J (or, Jansen) medium for Microcystis.

For 1 L Total pH 8.3 - 8.5

1. To approximately 950 mL of dH2O, add each of the components in the order specified while stirring continuously.
2. Bring total volume to 1 L with dH2O.
*For 1.25% agar medium add 12.5 g of agar into the flask; do not mix.
3. Cover and autoclave medium.
4. Store at refrigerator temperature.

# Component Amount Stock Solution
Concentration
Final Concentration
1 Macro Component 1 for J Medium 10 mL/L    
2 Macro Component 2 for J medium 10 mL/L    
3 CaCl2·2H2O (Sigma C-3881) 1 mL/L 0.29 g/100 mL dH20 0.02 mM
4 G9 Trace Metals for J medium 1 mL/L