J Medium



J Medium Recipe

Modification of Parker's J (or, Jansen) medium for Microcystis.

 

Directions

For 1 L Total pH 8.3 - 8.5

  1. To approximately 950 mL of dH2O,add each of the components in the order specified while stirring continuously.
  2. Bring total volume to 1 Liter with dH2O.
    • *For 1.25% (w/v) agar medium, add 12.5 grams of agar into the flask; do not mix.
  3. Cover and autoclave medium.
  4. Store at refrigerator temperature.

# Component Amount Stock Solution Concentration Final Concentration
1 Macro Component 1 for J Medium 10 mL/L    
2 Macro Component 2 for J medium 10 mL/L    
3 CaCl2·2H2O (Sigma C-3881) 1 mL/L 0.29 g/100 mL dH2O 0.02 mM
4 G9 Trace Metals for J medium 1 mL/L