Macro Component 2 for J Medium Recipe

To approximately 850 mL of dH₂O, add the following components in the order listed while stirring continuously.
Adjust the pH to 7.5.
Bring the total volume to 1 Liter with dH₂O.
Store at refrigerator temperature.

#	Component	Amount	Final Concentration
1	Na₂EDTA•2H₂O (Sigma ED255)	4.4 g/L	0.12 mM
2	MgSO₄•7H₂O (Sigma 230391)	10 g/L	0.41 mM
3	MgCl₂•6H₂O (Fisher M 33)	5 g/L	0.24 mM
4	FeCl₃•6H₂O (Sigma F 1513)	0.5 g/L	0.02 mM