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Macro Component 2 for J Medium Recipe

Directions

For 1 L Total

  1. To approximately 850 mL of dH2O, add the following components in the order listed while stirring continuously.
  2. Adjust the pH to 7.5.
  3. Bring the total volume to 1 Liter with dH2O.
  4. Store at refrigerator temperature.
# Component Amount Stock Solution Concentration Final Concentration
1 Na2EDTA•2H2O
(Sigma ED255)		 4.4 g/L 0.12 mM
2 MgSO4•7H2O
(Sigma 230391)		 10 g/L 0.41 mM
3 MgCl2•6H2O
(Fisher M 33)		 5 g/L 0.24 mM
4 FeCl3•6H2O
(Sigma F 1513)		 0.5 g/L 0.02 mM

Note: Final concentration listed is for the stock solution.