Macro Component 1 for J Medium Recipe

To approximately 900 mL of dH₂O, add the following components in the order listed while stirring continuously.
Adjust the pH 8.5 to 8.8.
Bring the total volume to 1 Liter with dH₂O.
Store at refrigerator temperature.

#	Component	Amount	Final Concentration
1	NaNO₃ (Fisher BP360)	67 g/L	7.9 mM
2	Na₂HPO₄ (Sigma S5136)	0.67 g/L	0.05 mM
3	K₂HPO₄ (Sigma P 3786)	3.8 g/L	0.22 mM
4	Tricine (Sigma T-5816-256)	25 g/L	1.4 mM