Macro Component 1 for J Medium Recipe

Directions

For 1 L Total

  1. To approximately 900 mL of dH2O, add the following components in the order listed while stirring continuously.
  2. Adjust the pH 8.5 to 8.8.
  3. Bring the total volume to 1 L with dH2O.
  4. Store at refrigerator temperature.
# Component Amount Stock Solution Concentration Final Concentration
1 NaNO3
(Fisher BP360-500)
67 g/L 7.9 mM
2 Na2HPO4
(Sigma S5136)
0.67 g/L 0.05 mM
3 K2HPO4
(Sigma P 3786)
3.8 g/L 0.22 mM
4 Tricine
(Sigma T-5816-256)
25 g/L 1.4 mM

Note: Final concentration listed is for the stock solution.