Macro Component 1 for J Medium Recipe
Directions
For 1 L Total
- To approximately 900 mL of dH2O, add the following components in the order listed while stirring continuously.
- Adjust the pH 8.5 to 8.8.
- Bring the total volume to 1 Liter with dH2O.
- Store at refrigerator temperature.
| # | Component | Amount | Stock Solution Concentration | Final Concentration |
|---|---|---|---|---|
| 1 | NaNO3 (Fisher BP360) |
67 g/L | 7.9 mM | |
| 2 | Na2HPO4 (Sigma S5136) |
0.67 g/L | 0.05 mM | |
| 3 | K2HPO4 (Sigma P 3786) |
3.8 g/L | 0.22 mM | |
| 4 | Tricine (Sigma T-5816-256) |
25 g/L | 1.4 mM |
Note: Final concentration listed is for the stock solution.
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