Erdschreiber's Medium



Erdschreiber's Medium Recipe

Directions

Modified from the original Plymouth seawater recipe. General purpose marine medium for xenic cultures [for bacteria-free cultures see reprints in Rosowski & Parker (1971)].

For 3 L Total

  1. To 3 L of pasteurized filtered seawater (30 ppt), aseptically add each of the sterile components in the order specified.
  2. Vigorously swirl the contents of the flask to mix thoroughly.
  3. Store at refrigerator temperature.

# Component Amount Stock Solution Concentration Final Concentration
1 Pasteurized Seawater 3 L
2 P-IV Metal Solution 36 mL/3 L
3 NaNO3 (autoclave before adding) (Fisher BP360-500) 10 mL/3 L 0.7 M 2.3 mM
4 Na2HPO4·7H2O (autoclave before adding) (Sigma S-9390) 10 mL/3 L 0.02 M 0.067 mM
5 Soilwater: GR+ Medium 150mL/3 L
6 Vitamin B12 Solution 3 mL/3 L