DYIII Medium



DYIII Medium Recipe

Directions 
Modification of Sandgren's recipe. Suitable for xenic cultures of freshwater chrysophytes.

For 1 L Total

1. To approximately 950 mL of dH2O add each of the components in the order specified (except vitamins) while stirring continuously.
2. Adjust the pH to 6.7.
3. Bring the total volume to 1 L with dH2O.
*For 1.5% agar medium add 15 g of agar into the flask; do not mix.
4. Cover and autoclave medium.
5. When cooled add vitamins.
*For agar medium add vitamins, mix, and dispense before agar solidifies.
6. Store at refrigerator temperature.

# Component Amount Stock Solution
Concentration
Final Concentration
1 MES (Sigma M-8250) 1.95 g/L   10 mM
2 MWC Metal Solution 10 mL/L    
3 CaCl2·2H2O (Sigma C-3881) 1 mL/L 2 g/100 mL 0.14 mM
4 MgSO4·7H2O (Sigma 230391) 1 mL/L 7.4 g/100 mL 0.3 mM
5 Na2glycerophosphate.5H2O (Sigma G 6501 ) 1 mL/L 1 g/100 mL 0.03 mM
6 NaNO3 (Fisher BP360-500) 1 mL/L 2 g/100 mL 0.24 mM
7 Na2SiO3·9H2O (Sigma 307815) 1 mL/L 1.5 g/100 mL 0.05 mM
8 NH4NO3 (Fisher A676) 1 mL/L 1 g/100 mL 0.12 mM
9 KCl (Fisher P 217) 1 mL/L 1 g/100 mL 0.13 mM
10 Vitamin B12 1 mL/L    
11 Biotin Vitamin Solution 1 mL/L    
12 Thiamine Vitamin Solution 1 mL/L