DYIII Medium



DYIII Medium Recipe

Modification of Sandgren's recipe. Suitable for xenic cultures of freshwater chrysophytes.

 

Directions

For 1 L Total

  1. To approximately 950 mL of dH2O add each of the components in the order specified (except vitamins) while stirring continuously.
  2. Adjust the pH to 6.7
  3. Bring total volume to 1 Liter with dH2O.
    • *For 1.5% agar medium, add 15 grams of agar into the flask; do not mix.
  4. Cover and autoclave medium.
  5. When cooled add vitamins.
    • *For agar medium add vitamins, mix, and dispense before agar solidifies.
  6. Store at refrigerator temperature.

# Component Amount Stock Solution Concentration Final Concentration
1 MES (Sigma M-8250) 1.95 g/L   10 mM
2 MWC Metal Solution 10 mL/L    
3 CaCl2·2H2O (Sigma C-3881) 1 mL/L 2 g/100 mL 0.14 mM
4 MgSO4·7H2O (Sigma 230391) 1 mL/L 7.4 g/100 mL 0.3 mM
5 Na2glycerophosphate.5H2O (Sigma G 6501 ) 1 mL/L 1 g/100 mL 0.03 mM
6 NaNO3 (Fisher BP360-500) 1 mL/L 2 g/100 mL 0.24 mM
7 Na2SiO3·9H2O (Sigma 307815) 1 mL/L 1.5 g/100 mL 0.05 mM
8 NH4NO3 (Fisher A676) 1 mL/L 1 g/100 mL 0.12 mM
9 KCl (Fisher P 217) 1 mL/L 1 g/100 mL 0.13 mM
10 Vitamin B12 1 mL/L    
11 Biotin Vitamin Solution 1 mL/L    
12 Thiamine Vitamin Solution 1 mL/L