2X Erdschreiber's Medium



2X Erdschreiber's Medium Recipe

Erdschreiber's Medium made with Supplemented Seawater instead of Pasteurized Seawater.

Directions

For 3 L Total

  1. To 3 L of pasteurized Supplemented Seawater (60 ppt), aseptically add each of the sterile components in the order specified.
  2. Vigorously swirl the contents of the flask to mix thoroughly.
  3. Store at refrigerator temperature.

# Component Amount Stock Solution Concentration Final Concentration
1 Supplemented Seawater 3 L
2 P-IV Metal Solution 36 mL
3 NaNO3 (autoclave before adding) (Fisher BP360-500) 10 mL 0.7 M 2.3 mM
4 Na2HPO4·7H2O (autoclave before adding) (Sigma S-9390) 10 mL 0.02 M 0.067 mM
5 Soilwater: GR+ Medium 150 mL
6 Vitamin B12 Solution 3 mL