UTEX B SP20
Dunaliella sp.


Condition: Xenic

Medium Type: Agar Slant


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View General Maintenance Conditions


Algae Details

UTEX Number: SP20
Class: Chlorophyceae
Strain: Dunaliella sp.
Medium: 5% F/2 Medium (5% F/2)
Origin: Great Salt Plains, Oklahoma, USA
Description of Location: soil
GPS: 36_ 43.911, -98_ 15.261
Type Culture: No
Collection: W.J. Henley, A.E. Kirkwood
Isolation: A.E. Kirkwood
Isolator Number:
Deposition: W.J. Henley (6/05)
Relatives: SPMO 210-3; CCMP 2605
Also Known As:
Notes:

About The Salt Plains (SP) Collection

The Salt Plains National Wildlife Refuge in northwest Oklahoma, USA is a moist soil habitat characterized by highly fluctuating salinity (ranging from freshwater to NaCl saturation) and temperature extremes (sometimes exceeding 50 °C). A broad range of halotolerant soil algae occur in this habitat, sometimes within microbial mats or in shallow pools.

A diverse range of Chlorophyceae, Bacillariophyceae and Cyanophyceae have been isolated and provided tentative identification by Dr. Andrea Kirkwood, and representative strains have been deposited in UTEX by Dr. William Henley. These strains have now been cryopreserved and are maintained at UTEX in indefinite storage under liquid nitrogen.

View other strains in this collection

General Long-Term Maintenance Conditions

Temperature: 20 °C
Light source: mix of warm-white & cool-white fluorescent lamps
Intensity: 3200 lux (maximum)
Periodicity: 12:12 Light/Dark Cycle

To reduce the risk of losing newly acquired cultures, it is highly recommended to initially duplicate the Collection's long-term maintenance conditions. Please note that these general maintenance conditions are not designed for optimal algal growth rates or large quantities. For information on the best growth conditions and media, consult other literature or conduct your own experiments. Once a stock culture is established, subcultures can be used to test other conditions.

You can find additional details on the long-term culture maintenance conditions on the Culture Maintenance Guides page.


Preparation of Living Algal Strains For Orders

Agar cultures are transferred to fresh agar slants in 20-mm glass screwcap tubes. After quality control (QC) checks via microscopy are completed, the tubes are cultured under a diurnal light cycle for a minimum of three (3) days.

Cultures are sealed and packaged in a temperature-regulating mailer and shipped the same day.

UTEX makes no attempt to quantify the number of organisms or other characteristics of the culture and only guarantees the identity of the organism as specified in the strain history.


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