UTEX B SNO73
Stichococcus sp.


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Algae Details

UTEX Number: SNO73
Class: Chlorophyceae
Strain: Stichococcus sp.
Medium: Soil Extract Medium (SE)
Origin: Mount Lemon, Arizona, USA
Description of Location:
GPS: elevation 2591-2791m, 8500-9157'
Type Culture: No
Collection: R.W. Hoshaw, R.W. Hoham (4/26/87)
Isolation: R.W. Hoham
Isolator Number: CU 565I
Deposition: R.W. Hoham (12/6/05)
Relatives:
Also Known As:
Notes:

The Snow Algae (SNO) Collection

The culture collection of snow algae maintained by Dr. Ron Hoham at Colgate University has been deposited in the Culture Collection of Algae at the University of Texas at Austin. The strains in this collection have all been isolated from snow and have been maintained at temperatures below 6 °C since their isolation. This collection includes isolates from eastern and western North America collected over a 35-year time period, and also includes isolates from Antarctica. Multiple isolates of the same taxon are maintained to permit research at the population level.

Most of the algae in this collection are members of Chlorophyta, but several strains are yellow-green algae (Xanthophyceae) and a few are golden algae (Chrysophyceae). Nearly all strains in the collection have been cryopreserved and in the future will be maintained at UTEX only at liquid nitrogen temperature.

These strains will survive transport at room temperature and that is the most reliable way to ship, but there is concern from the depositor that allowing them to warm during shipment may select for genotypes that are not as cold-adapted. Cultures will be shipped in a large styrofoam cooler box with ice pack.

General Maintenance Conditions

Temperature: 5 °C
Light source: mix of warm-white & cool-white fluorescent lamps
Intensity: 3200 lux (maximum)
Periodicity: 12/12 h L/D

Those who receive cultures are encouraged to duplicate within reason the conditions used in the Collection, when handling newly acquired cultures, to reduce the chance of losing the culture. After a stock culture is established, subcultures may be used for testing other conditions. These general maintenance conditions are not our recommendations to achieve optimal growth rates and large quantities of algae. Information on the best growth conditions and media must be acquired in other literature or through careful experimentation. For additional details on the long-term culture maintenance conditions utilized at UTEX, visit the Culture Maintenance Guides page.


Preparation of Living Algal Strains For Orders

For agar cultures, strains are transferred to the surface of fresh agar slants in 20 mm diamter glass screw-cap tubes in preparation for shipment. The fresh inocula are grown in a diurnal light cycle for at least 3 days until a macroscopically visible lawn appears.

Agar and liquid cultures are packaged within a Styrofoam box and shipped the same day they are packaged.

One tube of agar or liquid culture constitutes an order for a single culture of a single strain of living algae. UTEX makes no attempt to quantify the number of organisms or other characteristics of the culture. UTEX only guarantees the identity of the organism as specified in the strain history.


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