Soil Extract Medium

ATTENTION:

Due to the upcoming 2018 Winter Holiday closures, orders placed after the stated deadlines will not ship until 2019 (review expected shipping estimates).




Soil Extract Medium Recipe

General purpose freshwater medium suitable for axenic and xenic cultures. Modification of Bristol medium.

Directions

For 1 L Total

  1. Prepare 1 L of Bristol Medium and thoroughly mix.
  2. Discard 40 mL of the Bristol Medium.
  3. Add 40 mL of previously prepared Soilwater: GR+ Medium (Note: The GR+ should be filtered to remove soil particles].
    • *For 1.5% (w/v) agar medium: add 15 g of agar into the flask; do not mix.
  4. Cover and autoclave medium. 
  5. Store at refrigerator temperature.

# Component Amount Stock Solution Concentration Final Concentration
1 Bristol Medium 960 mL
2 Soilwater: GR+ Medium 40 mL of supernatant