Soil Extract Medium


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Soil Extract Medium Recipe

General purpose freshwater medium suitable for axenic and xenic cultures. Modification of Bristol medium.


For 1 L Total

  1. Prepare 1 L of Bristol Medium and thoroughly mix.
  2. Discard 40 mL of the Bristol Medium.
  3. Add 40 mL of previously prepared Soilwater: GR+ Medium (Note: The GR+ should be filtered to remove soil particles].
    • *For 1.5% (w/v) agar medium: add 15 g of agar into the flask; do not mix.
  4. Cover and autoclave medium. 
  5. Store at refrigerator temperature.

# Component Amount Stock Solution Concentration Final Concentration
1 Bristol Medium 960 mL
2 Soilwater: GR+ Medium 40 mL of supernatant