UTEX B 3195
Nodosilinea sp.


Deadline to place an order for UTEX B 3195 Nodosilinea sp. with a 2024 shipment date has passed. New orders will not ship until the 2025 new year—see announcement or order confirmation email for the expected ship date.




Algae Details

UTEX Number: 3195
Class: Cyanophyceae
Strain: Nodosilinea sp.
DNA Sequence: Type 16s rDNA | GenBank: OP142385
Media: BG-11 Medium (BG11)
Origin: Creston, Iowa, USA
Description of Location: Isolated from a Revolving Algal Biofilm (RAB) municipal wastewater treatment system
Type Culture: No
Collection: Michael Gross (04/13/2021)
Isolation: Eric Schaedig/Chris Urban/Michael Cantrell
Isolator Number: TCF-10c
Deposition: Eric Schaedig (10/14/2022)
Relatives:
Also Known As:
Notes: Accumulates polyphosphate; 16s rDNA sequence - Genbank: OP142385

General Maintenance Conditions

Temperature: 20 °C
Light source: mix of warm-white & cool-white fluorescent lamps
Intensity: 3200 lux (maximum)
Periodicity: 12/12 h L/D

Those who receive cultures are encouraged to duplicate within reason the conditions used in the Collection, when handling newly acquired cultures, to reduce the chance of losing the culture. After a stock culture is established, subcultures may be used for testing other conditions. These general maintenance conditions are not our recommendations to achieve optimal growth rates and large quantities of algae. Information on the best growth conditions and media must be acquired in other literature or through careful experimentation. For additional details on the long-term culture maintenance conditions utilized at UTEX, visit the Culture Maintenance Guides page.


Preparation of Living Algal Strains For Orders

For agar cultures, strains are transferred to the surface of fresh agar slants in 20 mm diamter glass screw-cap tubes in preparation for shipment. The fresh inocula are grown in a diurnal light cycle for at least 3 days until a macroscopically visible lawn appears.

Agar and liquid cultures are packaged within a Styrofoam box and shipped the same day they are packaged.

One tube of agar or liquid culture constitutes an order for a single culture of a single strain of living algae. UTEX makes no attempt to quantify the number of organisms or other characteristics of the culture. UTEX only guarantees the identity of the organism as specified in the strain history.


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