BG-11 Medium



BG-11 Medium Recipe

Improved recipe as of March 2009

Directions

For 1 L Total

Liquid media:

  1. To approximately 900 mL of dH2O, add the first nine (9) components in the order specified while stirring continuously.
  2. Bring the total volume to 1 L with dH2O.
    • Cover and autoclave medium.
    • Allow to cool then store at refrigerator temperature.

    Agar media:

    1. To approximately 400 mL of dH2O, add the first nine (9) components in the order specified while stirring continuously.
    2. Bring total volume to 500 mL with dH2O.
    3. In a separate container add 15 g of agar to 500 mL of dH2O.
    4. Cover and autoclave both solutions.
    5. In a water bath, allow both solutions to cool to 45 - 50 °C.
    6. Add sterile Sodium Thiosulfate (Component #10) to agar solution and mix well.
    7. Combine both agar and liquid solutions, mix well. Note the agar can solidify quickly.
    8. Allow to cool then store at refrigerator temperature.

    # Component Amount Stock Solution Concentration Final Concentration
    1 NaNO3 (Fisher BP360-500) 10 mL/L 30 g/200 mL dH2O 17.6 mM
    2 K2HPO4 (Sigma P 3786) 10 mL/L 0.8 g/200 mL dH2O 0.23 mM
    3 MgSO4·7H2O (Sigma 230391) 10 mL/L 1.5 g/200 mL dH2O 0.3 mM
    4 CaCl2·2H2O (Sigma C-3881) 10 mL/L 0.72 g/200 mL dH2O 0.24 mM
    5 Citric Acid·H2O (Fisher A 104) 10 mL/L 0.12 g/200 mL dH2O 0.031 mM
    6 Ferric Ammonium Citrate 10 mL/L 0.12 g/200 mL dH2O 0.021 mM
    7 Na2EDTA·2H2O (Sigma ED255) 10 mL/L 0.02 g/200 mL dH2O 0.0027 mM
    8 Na2CO3 (Baker 3604) 10 mL/L 0.4 g/200 mL dH2O 0.19 mM
    9 BG-11 Trace Metals Solution 1 mL/L
    10 Sodium Thiosulfate Pentahydrate (agar media only, sterile) (Baker 3946) 1 mL/L 49.6 g/200 mL dH2O 1 mM