Upcoming Ordering Deadline:

Orders placed after 5:00 PM CST on November 18th for Freshwater Diatom Collection strains (designated 'FD'), strains maintained only in cryopreservation (designated 'cryo only'), and bulk volume microalgae will not ship until early February 2020. Please place an order before the deadline if you would like the item to ship before the 2019 Winter Holiday Break closure.



UTEX B 775
Chlorococcum perforatum


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View Cryopreservation Conditions



Algae Details

UTEX Number: 775
Class: Chlorophyceae
Strain: Chlorococcum perforatum
DNA Sequence: Type -ITS | GenBank -KX147346 | Blast - KX147346
Medium: Soil Extract Medium (SE)
Origin: Cuba
Description of Location: coffee field soil
GPS:
Type Culture: Yes
Collection: J. Acuna (10/53)
Isolation: H.C. Bold, G. Arce
Isolator Number: 49B
Deposition: (1953-5)
Relatives:
Also Known As:
Notes: (Acre & Bold 1958)

Strains that are maintained only in cryopreservation (designated 'cryo only') require a minimum of 4-weeks to grow out before shipment.


Cryopreservation Conditions

Temperature: -190 °C
Storage: Vapor phase of liquid nitrogen dewars

Cryopreservation stabilizes genomic integrity, preserves culture quality, minimizes maintenance costs and reduces the risk of catastrophic loss. A broad variety of techniques employed at the CCAP, at UTEX, and elsewhere, can be found in Day, J.G. and Brand, J.J. Cryopreservation Methods for Maintaining Microalgal Cultures, in Algal Culturing Techniques, ed. R. A. Andersen, Elsevier, Amsterdam, 2006. A relatively simple method that uses a minimum of specialized equipment, yet allows a large variety of microalgae to be cryopreserved with high viability, can be found on the Cryopreservation of Microalgae page.


Preparation of Strains Maintained Only In Cryopreservation For Orders

Strains that are maintained only in cryopreservation (cryo only) require a minimum of 4-weeks to thaw, grow out, and check viability before shipment. An estimated shipping date will be emailed when purchased. Updates via email will be sent if the strain is ready sooner or needs to be delayed further.

One tube of agar or liquid culture constitutes an order for a single culture of a single strain of living algae. UTEX makes no attempt to quantify the number of organisms or other characteristics of the culture. UTEX only guarantees the identity of the organism as specified in the strain history.


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