UTEX B 481
Fremyella diplosiphon

UTEX Prefix Labeling System

LB Xenic strain maintained in liquid media
B Xenic strain maintained on an agar slant
'no prefix' Axenic strain, free of any contaminants and maintained on an agar slant


Algae Detail

UTEX Number: 481
Class: Cyanophyceae
Strain: Fremyella diplosiphon
Media: BG-11 Medium
Origin: New Haven, Connecticut, USA
Description of Location:
Type Culture: No
Collection:
Isolation: P.M. Strout (1952)
Isolator Number:
Deposition:
Relatives: CCAP 1429/1; SAG 1410-2 aka SAG 1410-1a [dec.]; PCC 7601 Tolypothrix (cluster 1) sp., PCC 7710 Tolypothrix (cluster 1) sp.; IAM M-100; ACMM 396; NIBB 1028
Also Known As: Calothrix sp.; Tolypothrix sp.
Notes: grown in shaded light; nitrogen fixation (Kratz & Myers 1955); plasmid DNA (Gendel 1988); contains restriction endonuclease Fdi (Streiner 1990); (Taylor & Barrow 1981 J. Clin. Path. 34:208)

General Maintenance Conditions

Temperature: 20 °C
Light source: cool-white fluorescent lamps
Intensity: 3200 lux (maximum)
Periodicity: 12/12 h L/D

Those who receive cultures are encouraged to duplicate within reason the conditions used in the Collection, when handling newly acquired cultures, to reduce the chance of losing the culture. After a stock culture is established, subcultures may be used for testing other conditions. These general maintenance conditions are not our recommendations to achieve optimal growth rates and large quantities of algae. Information on the best growth conditions and media must be acquired in other literature or through careful experimentation. For additional details on the long-term culture maintenance conditions utilized at UTEX, visit the Culture Maintenance Guides page.

Preparation of Living Algal Strains

Aliquots of algal strains that are maintained on agar slants are transferred to the surface of fresh slants in 20 mm diameter glass screw-captubes in preparation for shipment. The fresh inocula are grown in a diurnal light cycle for at least 3 days until a macroscopically visible lawn appears. Approximately 15 mL aliquots of strains that are maintained as liquid cultures are transferred to screw-cap tubes on the day of shipment. Agar and liquid cultures for a customer are packaged within a Styrofoam™ box and shipped on the same day they are packaged.

One tube of agar or liquid culture constitutes an order for a single culture of a single strain of living algae. UTEX makes no attempt to quantify the number of organisms or other characteristics of the culture. UTEX only guarantees the identity of the organism as specified in the strain history.

  • Algae Detail

    UTEX Number: 481
    Class: Cyanophyceae
    Strain: Fremyella diplosiphon
    Media: BG-11 Medium
    Origin: New Haven, Connecticut, USA
    Description of Location:
    Type Culture: No
    Collection:
    Isolation: P.M. Strout (1952)
    Isolator Number:
    Deposition:
    Relatives: CCAP 1429/1; SAG 1410-2 aka SAG 1410-1a [dec.]; PCC 7601 Tolypothrix (cluster 1) sp., PCC 7710 Tolypothrix (cluster 1) sp.; IAM M-100; ACMM 396; NIBB 1028
    Also Known As: Calothrix sp.; Tolypothrix sp.
    Notes: grown in shaded light; nitrogen fixation (Kratz & Myers 1955); plasmid DNA (Gendel 1988); contains restriction endonuclease Fdi (Streiner 1990); (Taylor & Barrow 1981 J. Clin. Path. 34:208)

  • General Maintenance Conditions

    Temperature: 20 °C
    Light source: cool-white fluorescent lamps
    Intensity: 3200 lux (maximum)
    Periodicity: 12/12 h L/D

    Those who receive cultures are encouraged to duplicate within reason the conditions used in the Collection, when handling newly acquired cultures, to reduce the chance of losing the culture. After a stock culture is established, subcultures may be used for testing other conditions. These general maintenance conditions are not our recommendations to achieve optimal growth rates and large quantities of algae. Information on the best growth conditions and media must be acquired in other literature or through careful experimentation. For additional details on the long-term culture maintenance conditions utilized at UTEX, visit the Culture Maintenance Guides page.


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